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TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214
Cancer stem cells are responsible for sustaining the tumor and giving rise to proliferating and progressively differentiating cells. However, the molecular mechanisms regulating the process of cancer stem cell differentiation is not clearly understood. Recently, we reported the isolation of the epit...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889129/ https://www.ncbi.nlm.nih.gov/pubmed/20400975 http://dx.doi.org/10.1038/onc.2010.111 |
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author | Yin, Gang Chen, Rui Alvero, Ayesha B. Fu, Han-Hsuan Holmberg, Jennie Glackin, Carlotta Rutherford, Thomas Mor, Gil |
author_facet | Yin, Gang Chen, Rui Alvero, Ayesha B. Fu, Han-Hsuan Holmberg, Jennie Glackin, Carlotta Rutherford, Thomas Mor, Gil |
author_sort | Yin, Gang |
collection | PubMed |
description | Cancer stem cells are responsible for sustaining the tumor and giving rise to proliferating and progressively differentiating cells. However, the molecular mechanisms regulating the process of cancer stem cell differentiation is not clearly understood. Recently, we reported the isolation of the epithelial ovarian cancer (EOC) stem cells (Type I/CD44+). In this study we show that Type I/CD44+ cells are characterized by low levels of both miR-199a and miR-214, whereas mature EOC cells (Type II/CD44-) have higher levels of miR-199a and miR-214. Moreover, these two miRNAs are regulated as a cluster on pri-miR-199a2 within the human Dnm3os gene (GenBank FJ623959). This study identify Twist1 as a regulator of this unique miRNA cluster responsible for the regulation of the IKKβ/NFκB and PTEN/AKT pathways and its association of ovarian cancer stem cell differentiation. Our data suggest that Twist1 may be an important regulator of “stemness” in EOC cells. The regulation of MIR199A2/214 expression may be used as a potential therapeutic approach in EOC patients. |
format | Text |
id | pubmed-2889129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
record_format | MEDLINE/PubMed |
spelling | pubmed-28891292010-12-01 TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 Yin, Gang Chen, Rui Alvero, Ayesha B. Fu, Han-Hsuan Holmberg, Jennie Glackin, Carlotta Rutherford, Thomas Mor, Gil Oncogene Article Cancer stem cells are responsible for sustaining the tumor and giving rise to proliferating and progressively differentiating cells. However, the molecular mechanisms regulating the process of cancer stem cell differentiation is not clearly understood. Recently, we reported the isolation of the epithelial ovarian cancer (EOC) stem cells (Type I/CD44+). In this study we show that Type I/CD44+ cells are characterized by low levels of both miR-199a and miR-214, whereas mature EOC cells (Type II/CD44-) have higher levels of miR-199a and miR-214. Moreover, these two miRNAs are regulated as a cluster on pri-miR-199a2 within the human Dnm3os gene (GenBank FJ623959). This study identify Twist1 as a regulator of this unique miRNA cluster responsible for the regulation of the IKKβ/NFκB and PTEN/AKT pathways and its association of ovarian cancer stem cell differentiation. Our data suggest that Twist1 may be an important regulator of “stemness” in EOC cells. The regulation of MIR199A2/214 expression may be used as a potential therapeutic approach in EOC patients. 2010-04-19 2010-06-17 /pmc/articles/PMC2889129/ /pubmed/20400975 http://dx.doi.org/10.1038/onc.2010.111 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Yin, Gang Chen, Rui Alvero, Ayesha B. Fu, Han-Hsuan Holmberg, Jennie Glackin, Carlotta Rutherford, Thomas Mor, Gil TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 |
title | TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 |
title_full | TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 |
title_fullStr | TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 |
title_full_unstemmed | TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 |
title_short | TWISTing Stemness, Inflammation, and Proliferation of Epithelial Ovarian Cancer Cells through MIR199A2/214 |
title_sort | twisting stemness, inflammation, and proliferation of epithelial ovarian cancer cells through mir199a2/214 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889129/ https://www.ncbi.nlm.nih.gov/pubmed/20400975 http://dx.doi.org/10.1038/onc.2010.111 |
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