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Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
BACKGROUND: Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chond...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889854/ https://www.ncbi.nlm.nih.gov/pubmed/20478023 http://dx.doi.org/10.1186/1475-2859-9-34 |
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author | Zanfardino, Anna Restaino, Odile F Notomista, Eugenio Cimini, Donatella Schiraldi, Chiara De Rosa, Mario De Felice, Maurilio Varcamonti, Mario |
author_facet | Zanfardino, Anna Restaino, Odile F Notomista, Eugenio Cimini, Donatella Schiraldi, Chiara De Rosa, Mario De Felice, Maurilio Varcamonti, Mario |
author_sort | Zanfardino, Anna |
collection | PubMed |
description | BACKGROUND: Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide. RESULTS: A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP), with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented. CONCLUSION: The enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide. |
format | Text |
id | pubmed-2889854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28898542010-06-23 Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin Zanfardino, Anna Restaino, Odile F Notomista, Eugenio Cimini, Donatella Schiraldi, Chiara De Rosa, Mario De Felice, Maurilio Varcamonti, Mario Microb Cell Fact Research BACKGROUND: Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide. RESULTS: A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP), with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented. CONCLUSION: The enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide. BioMed Central 2010-05-17 /pmc/articles/PMC2889854/ /pubmed/20478023 http://dx.doi.org/10.1186/1475-2859-9-34 Text en Copyright ©2010 Zanfardino et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Zanfardino, Anna Restaino, Odile F Notomista, Eugenio Cimini, Donatella Schiraldi, Chiara De Rosa, Mario De Felice, Maurilio Varcamonti, Mario Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin |
title | Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin |
title_full | Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin |
title_fullStr | Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin |
title_full_unstemmed | Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin |
title_short | Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin |
title_sort | isolation of an escherichia coli k4 kfoc mutant over-producing capsular chondroitin |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889854/ https://www.ncbi.nlm.nih.gov/pubmed/20478023 http://dx.doi.org/10.1186/1475-2859-9-34 |
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