Cargando…

Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin

BACKGROUND: Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chond...

Descripción completa

Detalles Bibliográficos
Autores principales: Zanfardino, Anna, Restaino, Odile F, Notomista, Eugenio, Cimini, Donatella, Schiraldi, Chiara, De Rosa, Mario, De Felice, Maurilio, Varcamonti, Mario
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889854/
https://www.ncbi.nlm.nih.gov/pubmed/20478023
http://dx.doi.org/10.1186/1475-2859-9-34
_version_ 1782182724907302912
author Zanfardino, Anna
Restaino, Odile F
Notomista, Eugenio
Cimini, Donatella
Schiraldi, Chiara
De Rosa, Mario
De Felice, Maurilio
Varcamonti, Mario
author_facet Zanfardino, Anna
Restaino, Odile F
Notomista, Eugenio
Cimini, Donatella
Schiraldi, Chiara
De Rosa, Mario
De Felice, Maurilio
Varcamonti, Mario
author_sort Zanfardino, Anna
collection PubMed
description BACKGROUND: Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide. RESULTS: A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP), with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented. CONCLUSION: The enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide.
format Text
id pubmed-2889854
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-28898542010-06-23 Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin Zanfardino, Anna Restaino, Odile F Notomista, Eugenio Cimini, Donatella Schiraldi, Chiara De Rosa, Mario De Felice, Maurilio Varcamonti, Mario Microb Cell Fact Research BACKGROUND: Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide. RESULTS: A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP), with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented. CONCLUSION: The enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide. BioMed Central 2010-05-17 /pmc/articles/PMC2889854/ /pubmed/20478023 http://dx.doi.org/10.1186/1475-2859-9-34 Text en Copyright ©2010 Zanfardino et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zanfardino, Anna
Restaino, Odile F
Notomista, Eugenio
Cimini, Donatella
Schiraldi, Chiara
De Rosa, Mario
De Felice, Maurilio
Varcamonti, Mario
Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
title Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
title_full Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
title_fullStr Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
title_full_unstemmed Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
title_short Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin
title_sort isolation of an escherichia coli k4 kfoc mutant over-producing capsular chondroitin
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889854/
https://www.ncbi.nlm.nih.gov/pubmed/20478023
http://dx.doi.org/10.1186/1475-2859-9-34
work_keys_str_mv AT zanfardinoanna isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT restainoodilef isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT notomistaeugenio isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT ciminidonatella isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT schiraldichiara isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT derosamario isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT defelicemaurilio isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin
AT varcamontimario isolationofanescherichiacolik4kfocmutantoverproducingcapsularchondroitin