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Characterization of H5N1 influenza viruses isolated from humans in vitro
Since December 1997, highly pathogenic avian influenza A H5N1viruses have swept through poultry populations across Asian countries and been transmitted into African and European countries. We characterized 6 avian influenza H5N1 viruses isolated from humans in 2004 in Thailand. A highly pathogenic (...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890526/ https://www.ncbi.nlm.nih.gov/pubmed/20515447 http://dx.doi.org/10.1186/1743-422X-7-112 |
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author | Li, Yong-Gang Chittaganpitch, Malinee Waicharoen, Sunthareeya Kanai, Yuta Bai, Gui-Rong Kameoka, Masanori Takeda, Naokazu Ikuta, Kazuyoshi Sawanpanyalert, Pathom |
author_facet | Li, Yong-Gang Chittaganpitch, Malinee Waicharoen, Sunthareeya Kanai, Yuta Bai, Gui-Rong Kameoka, Masanori Takeda, Naokazu Ikuta, Kazuyoshi Sawanpanyalert, Pathom |
author_sort | Li, Yong-Gang |
collection | PubMed |
description | Since December 1997, highly pathogenic avian influenza A H5N1viruses have swept through poultry populations across Asian countries and been transmitted into African and European countries. We characterized 6 avian influenza H5N1 viruses isolated from humans in 2004 in Thailand. A highly pathogenic (HP) KAN353 strain showed faster replication and higher virulence in embryonated eggs compared to other strains, especially compared to the low pathogenic (LP) SP83 strain. HP KAN353 also showed strong cytopathogenicity compared to SP83 in Madin-Darby canine kidney cells. Interestingly, LP SP83 induced smaller plaques compared to other strains, especially HP KAN353. PB2 amino acid 627E may contribute to low virulence, whereas either PB2 amino acid 627 K or the combination of 627E/701N seems to be associated with high virulence. The in vitro assays used in this study may provide the basis for assessing the pathogenesis of influenza H5N1 viruses in vivo. |
format | Text |
id | pubmed-2890526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28905262010-06-24 Characterization of H5N1 influenza viruses isolated from humans in vitro Li, Yong-Gang Chittaganpitch, Malinee Waicharoen, Sunthareeya Kanai, Yuta Bai, Gui-Rong Kameoka, Masanori Takeda, Naokazu Ikuta, Kazuyoshi Sawanpanyalert, Pathom Virol J Research Since December 1997, highly pathogenic avian influenza A H5N1viruses have swept through poultry populations across Asian countries and been transmitted into African and European countries. We characterized 6 avian influenza H5N1 viruses isolated from humans in 2004 in Thailand. A highly pathogenic (HP) KAN353 strain showed faster replication and higher virulence in embryonated eggs compared to other strains, especially compared to the low pathogenic (LP) SP83 strain. HP KAN353 also showed strong cytopathogenicity compared to SP83 in Madin-Darby canine kidney cells. Interestingly, LP SP83 induced smaller plaques compared to other strains, especially HP KAN353. PB2 amino acid 627E may contribute to low virulence, whereas either PB2 amino acid 627 K or the combination of 627E/701N seems to be associated with high virulence. The in vitro assays used in this study may provide the basis for assessing the pathogenesis of influenza H5N1 viruses in vivo. BioMed Central 2010-06-01 /pmc/articles/PMC2890526/ /pubmed/20515447 http://dx.doi.org/10.1186/1743-422X-7-112 Text en Copyright ©2010 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Li, Yong-Gang Chittaganpitch, Malinee Waicharoen, Sunthareeya Kanai, Yuta Bai, Gui-Rong Kameoka, Masanori Takeda, Naokazu Ikuta, Kazuyoshi Sawanpanyalert, Pathom Characterization of H5N1 influenza viruses isolated from humans in vitro |
title | Characterization of H5N1 influenza viruses isolated from humans in vitro |
title_full | Characterization of H5N1 influenza viruses isolated from humans in vitro |
title_fullStr | Characterization of H5N1 influenza viruses isolated from humans in vitro |
title_full_unstemmed | Characterization of H5N1 influenza viruses isolated from humans in vitro |
title_short | Characterization of H5N1 influenza viruses isolated from humans in vitro |
title_sort | characterization of h5n1 influenza viruses isolated from humans in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890526/ https://www.ncbi.nlm.nih.gov/pubmed/20515447 http://dx.doi.org/10.1186/1743-422X-7-112 |
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