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Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorde...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891991/ https://www.ncbi.nlm.nih.gov/pubmed/20585575 http://dx.doi.org/10.1371/journal.pone.0010964 |
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author | Cervelló, Irene Gil-Sanchis, Claudia Mas, Aymara Delgado-Rosas, Francisco Martínez-Conejero, José Antonio Galán, Amparo Martínez-Romero, Alicia Martínez, Sebastian Navarro, Ismael Ferro, Jaime Horcajadas, José Antonio Esteban, Francisco José O'Connor, José Enrique Pellicer, Antonio Simón, Carlos |
author_facet | Cervelló, Irene Gil-Sanchis, Claudia Mas, Aymara Delgado-Rosas, Francisco Martínez-Conejero, José Antonio Galán, Amparo Martínez-Romero, Alicia Martínez, Sebastian Navarro, Ismael Ferro, Jaime Horcajadas, José Antonio Esteban, Francisco José O'Connor, José Enrique Pellicer, Antonio Simón, Carlos |
author_sort | Cervelló, Irene |
collection | PubMed |
description | During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC. |
format | Text |
id | pubmed-2891991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28919912010-06-28 Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells Cervelló, Irene Gil-Sanchis, Claudia Mas, Aymara Delgado-Rosas, Francisco Martínez-Conejero, José Antonio Galán, Amparo Martínez-Romero, Alicia Martínez, Sebastian Navarro, Ismael Ferro, Jaime Horcajadas, José Antonio Esteban, Francisco José O'Connor, José Enrique Pellicer, Antonio Simón, Carlos PLoS One Research Article During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC. Public Library of Science 2010-06-24 /pmc/articles/PMC2891991/ /pubmed/20585575 http://dx.doi.org/10.1371/journal.pone.0010964 Text en Cervelló et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Cervelló, Irene Gil-Sanchis, Claudia Mas, Aymara Delgado-Rosas, Francisco Martínez-Conejero, José Antonio Galán, Amparo Martínez-Romero, Alicia Martínez, Sebastian Navarro, Ismael Ferro, Jaime Horcajadas, José Antonio Esteban, Francisco José O'Connor, José Enrique Pellicer, Antonio Simón, Carlos Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells |
title | Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells |
title_full | Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells |
title_fullStr | Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells |
title_full_unstemmed | Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells |
title_short | Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells |
title_sort | human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891991/ https://www.ncbi.nlm.nih.gov/pubmed/20585575 http://dx.doi.org/10.1371/journal.pone.0010964 |
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