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Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells

During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorde...

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Autores principales: Cervelló, Irene, Gil-Sanchis, Claudia, Mas, Aymara, Delgado-Rosas, Francisco, Martínez-Conejero, José Antonio, Galán, Amparo, Martínez-Romero, Alicia, Martínez, Sebastian, Navarro, Ismael, Ferro, Jaime, Horcajadas, José Antonio, Esteban, Francisco José, O'Connor, José Enrique, Pellicer, Antonio, Simón, Carlos
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891991/
https://www.ncbi.nlm.nih.gov/pubmed/20585575
http://dx.doi.org/10.1371/journal.pone.0010964
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author Cervelló, Irene
Gil-Sanchis, Claudia
Mas, Aymara
Delgado-Rosas, Francisco
Martínez-Conejero, José Antonio
Galán, Amparo
Martínez-Romero, Alicia
Martínez, Sebastian
Navarro, Ismael
Ferro, Jaime
Horcajadas, José Antonio
Esteban, Francisco José
O'Connor, José Enrique
Pellicer, Antonio
Simón, Carlos
author_facet Cervelló, Irene
Gil-Sanchis, Claudia
Mas, Aymara
Delgado-Rosas, Francisco
Martínez-Conejero, José Antonio
Galán, Amparo
Martínez-Romero, Alicia
Martínez, Sebastian
Navarro, Ismael
Ferro, Jaime
Horcajadas, José Antonio
Esteban, Francisco José
O'Connor, José Enrique
Pellicer, Antonio
Simón, Carlos
author_sort Cervelló, Irene
collection PubMed
description During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC.
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spelling pubmed-28919912010-06-28 Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells Cervelló, Irene Gil-Sanchis, Claudia Mas, Aymara Delgado-Rosas, Francisco Martínez-Conejero, José Antonio Galán, Amparo Martínez-Romero, Alicia Martínez, Sebastian Navarro, Ismael Ferro, Jaime Horcajadas, José Antonio Esteban, Francisco José O'Connor, José Enrique Pellicer, Antonio Simón, Carlos PLoS One Research Article During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC. Public Library of Science 2010-06-24 /pmc/articles/PMC2891991/ /pubmed/20585575 http://dx.doi.org/10.1371/journal.pone.0010964 Text en Cervelló et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cervelló, Irene
Gil-Sanchis, Claudia
Mas, Aymara
Delgado-Rosas, Francisco
Martínez-Conejero, José Antonio
Galán, Amparo
Martínez-Romero, Alicia
Martínez, Sebastian
Navarro, Ismael
Ferro, Jaime
Horcajadas, José Antonio
Esteban, Francisco José
O'Connor, José Enrique
Pellicer, Antonio
Simón, Carlos
Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
title Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
title_full Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
title_fullStr Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
title_full_unstemmed Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
title_short Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
title_sort human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891991/
https://www.ncbi.nlm.nih.gov/pubmed/20585575
http://dx.doi.org/10.1371/journal.pone.0010964
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