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A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns

Petal development and senescence entails a normally irreversible process. It starts with petal expansion and pigment production, and ends with nutrient remobilization and ultimately cell death. In many species this is accompanied by petal abscission. Post-harvest stress is an important factor in lim...

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Autores principales: Wagstaff, Carol, Bramke, Irene, Breeze, Emily, Thornber, Sarah, Harrison, Elizabeth, Thomas, Brian, Buchanan-Wollaston, Vicky, Stead, Tony, Rogers, Hilary
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892140/
https://www.ncbi.nlm.nih.gov/pubmed/20457576
http://dx.doi.org/10.1093/jxb/erq113
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author Wagstaff, Carol
Bramke, Irene
Breeze, Emily
Thornber, Sarah
Harrison, Elizabeth
Thomas, Brian
Buchanan-Wollaston, Vicky
Stead, Tony
Rogers, Hilary
author_facet Wagstaff, Carol
Bramke, Irene
Breeze, Emily
Thornber, Sarah
Harrison, Elizabeth
Thomas, Brian
Buchanan-Wollaston, Vicky
Stead, Tony
Rogers, Hilary
author_sort Wagstaff, Carol
collection PubMed
description Petal development and senescence entails a normally irreversible process. It starts with petal expansion and pigment production, and ends with nutrient remobilization and ultimately cell death. In many species this is accompanied by petal abscission. Post-harvest stress is an important factor in limiting petal longevity in cut flowers and accelerates some of the processes of senescence such as petal wilting and abscission. However, some of the effects of moderate stress in young flowers are reversible with appropriate treatments. Transcriptomic studies have shown that distinct gene sets are expressed during petal development and senescence. Despite this, the overlap in gene expression between developmental and stress-induced senescence in petals has not been fully investigated in any species. Here a custom-made cDNA microarray from Alstroemeria petals was used to investigate the overlap in gene expression between developmental changes (bud to first sign of senescence) and typical post-harvest stress treatments. Young flowers were stressed by cold or ambient temperatures without water followed by a recovery and rehydration period. Stressed flowers were still at the bud stage after stress treatments. Microarray analysis showed that ambient dehydration stress accelerates many of the changes in gene expression patterns that would normally occur during developmental senescence. However, a higher proportion of gene expression changes in response to cold stress were specific to this stimulus and not senescence related. The expression of 21 transcription factors was characterized, showing that overlapping sets of regulatory genes are activated during developmental senescence and by different stresses.
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spelling pubmed-28921402010-06-28 A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns Wagstaff, Carol Bramke, Irene Breeze, Emily Thornber, Sarah Harrison, Elizabeth Thomas, Brian Buchanan-Wollaston, Vicky Stead, Tony Rogers, Hilary J Exp Bot Research Papers Petal development and senescence entails a normally irreversible process. It starts with petal expansion and pigment production, and ends with nutrient remobilization and ultimately cell death. In many species this is accompanied by petal abscission. Post-harvest stress is an important factor in limiting petal longevity in cut flowers and accelerates some of the processes of senescence such as petal wilting and abscission. However, some of the effects of moderate stress in young flowers are reversible with appropriate treatments. Transcriptomic studies have shown that distinct gene sets are expressed during petal development and senescence. Despite this, the overlap in gene expression between developmental and stress-induced senescence in petals has not been fully investigated in any species. Here a custom-made cDNA microarray from Alstroemeria petals was used to investigate the overlap in gene expression between developmental changes (bud to first sign of senescence) and typical post-harvest stress treatments. Young flowers were stressed by cold or ambient temperatures without water followed by a recovery and rehydration period. Stressed flowers were still at the bud stage after stress treatments. Microarray analysis showed that ambient dehydration stress accelerates many of the changes in gene expression patterns that would normally occur during developmental senescence. However, a higher proportion of gene expression changes in response to cold stress were specific to this stimulus and not senescence related. The expression of 21 transcription factors was characterized, showing that overlapping sets of regulatory genes are activated during developmental senescence and by different stresses. Oxford University Press 2010-06 2010-05-09 /pmc/articles/PMC2892140/ /pubmed/20457576 http://dx.doi.org/10.1093/jxb/erq113 Text en © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Wagstaff, Carol
Bramke, Irene
Breeze, Emily
Thornber, Sarah
Harrison, Elizabeth
Thomas, Brian
Buchanan-Wollaston, Vicky
Stead, Tony
Rogers, Hilary
A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
title A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
title_full A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
title_fullStr A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
title_full_unstemmed A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
title_short A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
title_sort specific group of genes respond to cold dehydration stress in cut alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892140/
https://www.ncbi.nlm.nih.gov/pubmed/20457576
http://dx.doi.org/10.1093/jxb/erq113
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