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MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*

We examined the in vitro anti-human immunodeficiency virus (HIV) activity of MRN-100, an iron-based compound derived from bivalent and tervalent ferrates. MRN-100 action against HIV-1 (SF strain) was tested in primary cultures of peripheral blood mononuclear cells (MNC) by analyzing p24 antigen prod...

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Autores principales: Ghoneum, Mamdooh, Shaheen, Magda
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892344/
https://www.ncbi.nlm.nih.gov/pubmed/18955328
http://dx.doi.org/10.1093/ecam/nen019
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author Ghoneum, Mamdooh
Shaheen, Magda
author_facet Ghoneum, Mamdooh
Shaheen, Magda
author_sort Ghoneum, Mamdooh
collection PubMed
description We examined the in vitro anti-human immunodeficiency virus (HIV) activity of MRN-100, an iron-based compound derived from bivalent and tervalent ferrates. MRN-100 action against HIV-1 (SF strain) was tested in primary cultures of peripheral blood mononuclear cells (MNC) by analyzing p24 antigen production and percent survival of MNC infected with HIV. MRN-100 at a concentration of 10% (v/v) inhibited HIV-1 replication in 11 out of 14 samples (79%). The percentage of suppression of p24 antigen was −12.3 to 100% at 10 days post-treatment. MRN-100 also exhibited a significant protective effect in the survival of HIV-1-infected MNC. MNC survival post-treatment was dose dependent, 70.4% ± 8.4, 83.6% ± 10.7 and 90% ± 11.4, at concentrations 2.5, 5 and 10% (v/v), respectively, as compared with 53% ± 4 for HIV-1-infected MNC without treatment. The effect was detected as early as 4 days and continued up to 11 days. Treatment with MRN-100 caused no significant change in proliferative response of MNC alone or cocultured with different mitogens: PHA and Con-A (activators of T cell function) and PWM (activator of CD4(+) T cell-dependent B cells). We concluded that MRN-100 possesses anti-HIV activity in vitro and without an increase in lymphocyte proliferation, MRN-100 may be a useful agent for treating patients with acquired immunodeficiency syndrome.
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spelling pubmed-28923442010-07-29 MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro* Ghoneum, Mamdooh Shaheen, Magda Evid Based Complement Alternat Med Original Articles - Basic Science We examined the in vitro anti-human immunodeficiency virus (HIV) activity of MRN-100, an iron-based compound derived from bivalent and tervalent ferrates. MRN-100 action against HIV-1 (SF strain) was tested in primary cultures of peripheral blood mononuclear cells (MNC) by analyzing p24 antigen production and percent survival of MNC infected with HIV. MRN-100 at a concentration of 10% (v/v) inhibited HIV-1 replication in 11 out of 14 samples (79%). The percentage of suppression of p24 antigen was −12.3 to 100% at 10 days post-treatment. MRN-100 also exhibited a significant protective effect in the survival of HIV-1-infected MNC. MNC survival post-treatment was dose dependent, 70.4% ± 8.4, 83.6% ± 10.7 and 90% ± 11.4, at concentrations 2.5, 5 and 10% (v/v), respectively, as compared with 53% ± 4 for HIV-1-infected MNC without treatment. The effect was detected as early as 4 days and continued up to 11 days. Treatment with MRN-100 caused no significant change in proliferative response of MNC alone or cocultured with different mitogens: PHA and Con-A (activators of T cell function) and PWM (activator of CD4(+) T cell-dependent B cells). We concluded that MRN-100 possesses anti-HIV activity in vitro and without an increase in lymphocyte proliferation, MRN-100 may be a useful agent for treating patients with acquired immunodeficiency syndrome. Oxford University Press 2010-12 2008-03-20 /pmc/articles/PMC2892344/ /pubmed/18955328 http://dx.doi.org/10.1093/ecam/nen019 Text en © 2008 The Author(s). http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles - Basic Science
Ghoneum, Mamdooh
Shaheen, Magda
MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*
title MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*
title_full MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*
title_fullStr MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*
title_full_unstemmed MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*
title_short MRN-100, an Iron-based Compound, Possesses Anti-HIV Activity In Vitro*
title_sort mrn-100, an iron-based compound, possesses anti-hiv activity in vitro*
topic Original Articles - Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892344/
https://www.ncbi.nlm.nih.gov/pubmed/18955328
http://dx.doi.org/10.1093/ecam/nen019
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