Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation

Spatial compression among the longer DNA fragments occurs during DNA electrophoresis in agarose and non-agarose gels when using certain ions in the conductive buffer, impairing the range of fragment sizes resolved well in a single gel. Substitutions using various polyhydroxyl anions supported the un...

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Detalles Bibliográficos
Autores principales: Singhal, Hari, Ren, Yunzhao R., Kern, Scott E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892876/
https://www.ncbi.nlm.nih.gov/pubmed/20593002
http://dx.doi.org/10.1371/journal.pone.0011318
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author Singhal, Hari
Ren, Yunzhao R.
Kern, Scott E.
author_facet Singhal, Hari
Ren, Yunzhao R.
Kern, Scott E.
author_sort Singhal, Hari
collection PubMed
description Spatial compression among the longer DNA fragments occurs during DNA electrophoresis in agarose and non-agarose gels when using certain ions in the conductive buffer, impairing the range of fragment sizes resolved well in a single gel. Substitutions using various polyhydroxyl anions supported the underlying phenomenon as the complexation of Lewis acids to DNA. We saw significant improvements using conditions (lithium borate 10 mM cations, pH 6.5) favoring the formation of borate polyanions and having lower conductance and Joule heating, delayed electrolyte exhaustion, faster electrophoretic run-speed, and sharper separation of DNA bands from 100bp to 12 kb in a single run.
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spelling pubmed-28928762010-06-30 Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation Singhal, Hari Ren, Yunzhao R. Kern, Scott E. PLoS One Research Article Spatial compression among the longer DNA fragments occurs during DNA electrophoresis in agarose and non-agarose gels when using certain ions in the conductive buffer, impairing the range of fragment sizes resolved well in a single gel. Substitutions using various polyhydroxyl anions supported the underlying phenomenon as the complexation of Lewis acids to DNA. We saw significant improvements using conditions (lithium borate 10 mM cations, pH 6.5) favoring the formation of borate polyanions and having lower conductance and Joule heating, delayed electrolyte exhaustion, faster electrophoretic run-speed, and sharper separation of DNA bands from 100bp to 12 kb in a single run. Public Library of Science 2010-06-25 /pmc/articles/PMC2892876/ /pubmed/20593002 http://dx.doi.org/10.1371/journal.pone.0011318 Text en Singhal et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Singhal, Hari
Ren, Yunzhao R.
Kern, Scott E.
Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation
title Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation
title_full Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation
title_fullStr Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation
title_full_unstemmed Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation
title_short Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation
title_sort improved dna electrophoresis in conditions favoring polyborates and lewis acid complexation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892876/
https://www.ncbi.nlm.nih.gov/pubmed/20593002
http://dx.doi.org/10.1371/journal.pone.0011318
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