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Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal

BACKGROUND: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that desig...

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Autores principales: Guitard, Juliette, Andersen, Pernille, Ermont, Caroline, Gnidehou, Sédami, Fievet, Nadine, Lund, Ole, Deloron, Philippe, Ndam, Nicaise Tuikue
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893538/
https://www.ncbi.nlm.nih.gov/pubmed/20553578
http://dx.doi.org/10.1186/1475-2875-9-165
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author Guitard, Juliette
Andersen, Pernille
Ermont, Caroline
Gnidehou, Sédami
Fievet, Nadine
Lund, Ole
Deloron, Philippe
Ndam, Nicaise Tuikue
author_facet Guitard, Juliette
Andersen, Pernille
Ermont, Caroline
Gnidehou, Sédami
Fievet, Nadine
Lund, Ole
Deloron, Philippe
Ndam, Nicaise Tuikue
author_sort Guitard, Juliette
collection PubMed
description BACKGROUND: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. METHODS: The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5ε + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. RESULTS: In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. CONCLUSIONS: Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5ε domain, suggest its potential in inducing antibodies with broad reactivity.
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spelling pubmed-28935382010-06-30 Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal Guitard, Juliette Andersen, Pernille Ermont, Caroline Gnidehou, Sédami Fievet, Nadine Lund, Ole Deloron, Philippe Ndam, Nicaise Tuikue Malar J Research BACKGROUND: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. METHODS: The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5ε + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. RESULTS: In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. CONCLUSIONS: Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5ε domain, suggest its potential in inducing antibodies with broad reactivity. BioMed Central 2010-06-16 /pmc/articles/PMC2893538/ /pubmed/20553578 http://dx.doi.org/10.1186/1475-2875-9-165 Text en Copyright ©2010 Guitard et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Guitard, Juliette
Andersen, Pernille
Ermont, Caroline
Gnidehou, Sédami
Fievet, Nadine
Lund, Ole
Deloron, Philippe
Ndam, Nicaise Tuikue
Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal
title Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal
title_full Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal
title_fullStr Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal
title_full_unstemmed Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal
title_short Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal
title_sort plasmodium falciparum population dynamics in a cohort of pregnant women in senegal
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893538/
https://www.ncbi.nlm.nih.gov/pubmed/20553578
http://dx.doi.org/10.1186/1475-2875-9-165
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