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Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling

The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with...

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Detalles Bibliográficos
Autores principales: Block, Ethan R., Tolino, Michael A., Lozano, Jennifer S., Lathrop, Kira L., Sullenberger, Rebecca S., Mazie, Abigail R., Klarlund, Jes K.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893982/
https://www.ncbi.nlm.nih.gov/pubmed/20462956
http://dx.doi.org/10.1091/mbc.E09-12-1026
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author Block, Ethan R.
Tolino, Michael A.
Lozano, Jennifer S.
Lathrop, Kira L.
Sullenberger, Rebecca S.
Mazie, Abigail R.
Klarlund, Jes K.
author_facet Block, Ethan R.
Tolino, Michael A.
Lozano, Jennifer S.
Lathrop, Kira L.
Sullenberger, Rebecca S.
Mazie, Abigail R.
Klarlund, Jes K.
author_sort Block, Ethan R.
collection PubMed
description The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with adjacent extracellular matrix, and breakdown of physical separation of ligands from their receptors. Depending on the exact nature of wounds, some cues may be present only transiently or insignificantly. In many epithelia, activation of the epidermal growth factor receptor (EGFR) is a central event in induction of motility, and we find that its continuous activation is required for progression of healing of wounds in sheets of corneal epithelial cells. Here, we examine the hypothesis that edges, which are universally and continuously present in wounds, are a cue. Using a novel culture model we find that their presence is sufficient to cause activation of the EGFR and increased motility of cells in the absence of other cues. Edges that are bordered by agarose do not induce activation of the EGFR, indicating that activation is not due to loss of any specific type of cell–cell interaction but rather due to loss of physical constraints.
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spelling pubmed-28939822010-09-16 Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling Block, Ethan R. Tolino, Michael A. Lozano, Jennifer S. Lathrop, Kira L. Sullenberger, Rebecca S. Mazie, Abigail R. Klarlund, Jes K. Mol Biol Cell Articles The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with adjacent extracellular matrix, and breakdown of physical separation of ligands from their receptors. Depending on the exact nature of wounds, some cues may be present only transiently or insignificantly. In many epithelia, activation of the epidermal growth factor receptor (EGFR) is a central event in induction of motility, and we find that its continuous activation is required for progression of healing of wounds in sheets of corneal epithelial cells. Here, we examine the hypothesis that edges, which are universally and continuously present in wounds, are a cue. Using a novel culture model we find that their presence is sufficient to cause activation of the EGFR and increased motility of cells in the absence of other cues. Edges that are bordered by agarose do not induce activation of the EGFR, indicating that activation is not due to loss of any specific type of cell–cell interaction but rather due to loss of physical constraints. The American Society for Cell Biology 2010-07-01 /pmc/articles/PMC2893982/ /pubmed/20462956 http://dx.doi.org/10.1091/mbc.E09-12-1026 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Block, Ethan R.
Tolino, Michael A.
Lozano, Jennifer S.
Lathrop, Kira L.
Sullenberger, Rebecca S.
Mazie, Abigail R.
Klarlund, Jes K.
Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
title Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
title_full Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
title_fullStr Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
title_full_unstemmed Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
title_short Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
title_sort free edges in epithelial cell sheets stimulate epidermal growth factor receptor signaling
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893982/
https://www.ncbi.nlm.nih.gov/pubmed/20462956
http://dx.doi.org/10.1091/mbc.E09-12-1026
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