Cargando…
Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893982/ https://www.ncbi.nlm.nih.gov/pubmed/20462956 http://dx.doi.org/10.1091/mbc.E09-12-1026 |
_version_ | 1782183109675974656 |
---|---|
author | Block, Ethan R. Tolino, Michael A. Lozano, Jennifer S. Lathrop, Kira L. Sullenberger, Rebecca S. Mazie, Abigail R. Klarlund, Jes K. |
author_facet | Block, Ethan R. Tolino, Michael A. Lozano, Jennifer S. Lathrop, Kira L. Sullenberger, Rebecca S. Mazie, Abigail R. Klarlund, Jes K. |
author_sort | Block, Ethan R. |
collection | PubMed |
description | The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with adjacent extracellular matrix, and breakdown of physical separation of ligands from their receptors. Depending on the exact nature of wounds, some cues may be present only transiently or insignificantly. In many epithelia, activation of the epidermal growth factor receptor (EGFR) is a central event in induction of motility, and we find that its continuous activation is required for progression of healing of wounds in sheets of corneal epithelial cells. Here, we examine the hypothesis that edges, which are universally and continuously present in wounds, are a cue. Using a novel culture model we find that their presence is sufficient to cause activation of the EGFR and increased motility of cells in the absence of other cues. Edges that are bordered by agarose do not induce activation of the EGFR, indicating that activation is not due to loss of any specific type of cell–cell interaction but rather due to loss of physical constraints. |
format | Text |
id | pubmed-2893982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28939822010-09-16 Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling Block, Ethan R. Tolino, Michael A. Lozano, Jennifer S. Lathrop, Kira L. Sullenberger, Rebecca S. Mazie, Abigail R. Klarlund, Jes K. Mol Biol Cell Articles The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with adjacent extracellular matrix, and breakdown of physical separation of ligands from their receptors. Depending on the exact nature of wounds, some cues may be present only transiently or insignificantly. In many epithelia, activation of the epidermal growth factor receptor (EGFR) is a central event in induction of motility, and we find that its continuous activation is required for progression of healing of wounds in sheets of corneal epithelial cells. Here, we examine the hypothesis that edges, which are universally and continuously present in wounds, are a cue. Using a novel culture model we find that their presence is sufficient to cause activation of the EGFR and increased motility of cells in the absence of other cues. Edges that are bordered by agarose do not induce activation of the EGFR, indicating that activation is not due to loss of any specific type of cell–cell interaction but rather due to loss of physical constraints. The American Society for Cell Biology 2010-07-01 /pmc/articles/PMC2893982/ /pubmed/20462956 http://dx.doi.org/10.1091/mbc.E09-12-1026 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Block, Ethan R. Tolino, Michael A. Lozano, Jennifer S. Lathrop, Kira L. Sullenberger, Rebecca S. Mazie, Abigail R. Klarlund, Jes K. Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling |
title | Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling |
title_full | Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling |
title_fullStr | Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling |
title_full_unstemmed | Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling |
title_short | Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling |
title_sort | free edges in epithelial cell sheets stimulate epidermal growth factor receptor signaling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893982/ https://www.ncbi.nlm.nih.gov/pubmed/20462956 http://dx.doi.org/10.1091/mbc.E09-12-1026 |
work_keys_str_mv | AT blockethanr freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling AT tolinomichaela freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling AT lozanojennifers freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling AT lathropkiral freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling AT sullenbergerrebeccas freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling AT mazieabigailr freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling AT klarlundjesk freeedgesinepithelialcellsheetsstimulateepidermalgrowthfactorreceptorsignaling |