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Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae

In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issu...

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Autores principales: Geng, Jiefei, Nair, Usha, Yasumura-Yorimitsu, Kyoko, Klionsky, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893989/
https://www.ncbi.nlm.nih.gov/pubmed/20444978
http://dx.doi.org/10.1091/mbc.E09-11-0969
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author Geng, Jiefei
Nair, Usha
Yasumura-Yorimitsu, Kyoko
Klionsky, Daniel J.
author_facet Geng, Jiefei
Nair, Usha
Yasumura-Yorimitsu, Kyoko
Klionsky, Daniel J.
author_sort Geng, Jiefei
collection PubMed
description In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issue that has not been resolved is the origin of the lipids that form the autophagosome, the sequestering vesicle that is critical for autophagy. Here, we report that two post-Golgi proteins, Sec2 and Sec4, are required for autophagy. Sec4 is a Rab family GTPase, and Sec2 is its guanine nucleotide exchange factor. In sec2 and sec4 conditional mutant yeast, the anterograde movement of Atg9, a proposed membrane carrier, is impaired during starvation conditions. Similarly, in the sec2 mutant, Atg8 is inefficiently recruited to the phagophore assembly site, which is involved in autophagosome biogenesis, resulting in the generation of fewer autophagosomes. We propose that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation.
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spelling pubmed-28939892010-09-16 Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae Geng, Jiefei Nair, Usha Yasumura-Yorimitsu, Kyoko Klionsky, Daniel J. Mol Biol Cell Articles In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issue that has not been resolved is the origin of the lipids that form the autophagosome, the sequestering vesicle that is critical for autophagy. Here, we report that two post-Golgi proteins, Sec2 and Sec4, are required for autophagy. Sec4 is a Rab family GTPase, and Sec2 is its guanine nucleotide exchange factor. In sec2 and sec4 conditional mutant yeast, the anterograde movement of Atg9, a proposed membrane carrier, is impaired during starvation conditions. Similarly, in the sec2 mutant, Atg8 is inefficiently recruited to the phagophore assembly site, which is involved in autophagosome biogenesis, resulting in the generation of fewer autophagosomes. We propose that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation. The American Society for Cell Biology 2010-07-01 /pmc/articles/PMC2893989/ /pubmed/20444978 http://dx.doi.org/10.1091/mbc.E09-11-0969 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Geng, Jiefei
Nair, Usha
Yasumura-Yorimitsu, Kyoko
Klionsky, Daniel J.
Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
title Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
title_full Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
title_fullStr Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
title_full_unstemmed Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
title_short Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
title_sort post-golgi sec proteins are required for autophagy in saccharomyces cerevisiae
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893989/
https://www.ncbi.nlm.nih.gov/pubmed/20444978
http://dx.doi.org/10.1091/mbc.E09-11-0969
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