Cargando…
Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issu...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893989/ https://www.ncbi.nlm.nih.gov/pubmed/20444978 http://dx.doi.org/10.1091/mbc.E09-11-0969 |
_version_ | 1782183111390396416 |
---|---|
author | Geng, Jiefei Nair, Usha Yasumura-Yorimitsu, Kyoko Klionsky, Daniel J. |
author_facet | Geng, Jiefei Nair, Usha Yasumura-Yorimitsu, Kyoko Klionsky, Daniel J. |
author_sort | Geng, Jiefei |
collection | PubMed |
description | In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issue that has not been resolved is the origin of the lipids that form the autophagosome, the sequestering vesicle that is critical for autophagy. Here, we report that two post-Golgi proteins, Sec2 and Sec4, are required for autophagy. Sec4 is a Rab family GTPase, and Sec2 is its guanine nucleotide exchange factor. In sec2 and sec4 conditional mutant yeast, the anterograde movement of Atg9, a proposed membrane carrier, is impaired during starvation conditions. Similarly, in the sec2 mutant, Atg8 is inefficiently recruited to the phagophore assembly site, which is involved in autophagosome biogenesis, resulting in the generation of fewer autophagosomes. We propose that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation. |
format | Text |
id | pubmed-2893989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28939892010-09-16 Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae Geng, Jiefei Nair, Usha Yasumura-Yorimitsu, Kyoko Klionsky, Daniel J. Mol Biol Cell Articles In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issue that has not been resolved is the origin of the lipids that form the autophagosome, the sequestering vesicle that is critical for autophagy. Here, we report that two post-Golgi proteins, Sec2 and Sec4, are required for autophagy. Sec4 is a Rab family GTPase, and Sec2 is its guanine nucleotide exchange factor. In sec2 and sec4 conditional mutant yeast, the anterograde movement of Atg9, a proposed membrane carrier, is impaired during starvation conditions. Similarly, in the sec2 mutant, Atg8 is inefficiently recruited to the phagophore assembly site, which is involved in autophagosome biogenesis, resulting in the generation of fewer autophagosomes. We propose that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation. The American Society for Cell Biology 2010-07-01 /pmc/articles/PMC2893989/ /pubmed/20444978 http://dx.doi.org/10.1091/mbc.E09-11-0969 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Geng, Jiefei Nair, Usha Yasumura-Yorimitsu, Kyoko Klionsky, Daniel J. Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae |
title | Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae |
title_full | Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae |
title_fullStr | Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae |
title_full_unstemmed | Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae |
title_short | Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae |
title_sort | post-golgi sec proteins are required for autophagy in saccharomyces cerevisiae |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893989/ https://www.ncbi.nlm.nih.gov/pubmed/20444978 http://dx.doi.org/10.1091/mbc.E09-11-0969 |
work_keys_str_mv | AT gengjiefei postgolgisecproteinsarerequiredforautophagyinsaccharomycescerevisiae AT nairusha postgolgisecproteinsarerequiredforautophagyinsaccharomycescerevisiae AT yasumurayorimitsukyoko postgolgisecproteinsarerequiredforautophagyinsaccharomycescerevisiae AT klionskydanielj postgolgisecproteinsarerequiredforautophagyinsaccharomycescerevisiae |