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Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability
Bioconjugates made up of an enzyme and gold nanorods (GNRs) were fabricated by electrostatic interactions (layer-by-layer method, LBL) between anionic glucose oxidase (GOD) and positively charged GNRs. The assembled processes were monitored by UV–Vis spectra, zeta potential measurements, and transmi...
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Formato: | Texto |
Lenguaje: | English |
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Springer
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894096/ https://www.ncbi.nlm.nih.gov/pubmed/20596428 http://dx.doi.org/10.1007/s11671-009-9385-8 |
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author | Ma, Zhanfang Ding, Teng |
author_facet | Ma, Zhanfang Ding, Teng |
author_sort | Ma, Zhanfang |
collection | PubMed |
description | Bioconjugates made up of an enzyme and gold nanorods (GNRs) were fabricated by electrostatic interactions (layer-by-layer method, LBL) between anionic glucose oxidase (GOD) and positively charged GNRs. The assembled processes were monitored by UV–Vis spectra, zeta potential measurements, and transmission electron microscopy. The enzyme activity assays of the obtained bioconjugates display a relatively enhanced thermostability behavior in contrast with that of free enzyme. Free GOD in solution only retains about 22% of its relative activity at 90 °C. Unexpectedly, the immobilized GOD on GNRs still retains about 39.3% activity after the same treatment. This work will be of significance for the biologic enhancement using other kinds of anisotropic nanostructure and suggests a new way of enhancing enzyme thermostability using anisotropic metal nanomaterials. |
format | Text |
id | pubmed-2894096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-28940962010-06-30 Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability Ma, Zhanfang Ding, Teng Nanoscale Res Lett Nano Express Bioconjugates made up of an enzyme and gold nanorods (GNRs) were fabricated by electrostatic interactions (layer-by-layer method, LBL) between anionic glucose oxidase (GOD) and positively charged GNRs. The assembled processes were monitored by UV–Vis spectra, zeta potential measurements, and transmission electron microscopy. The enzyme activity assays of the obtained bioconjugates display a relatively enhanced thermostability behavior in contrast with that of free enzyme. Free GOD in solution only retains about 22% of its relative activity at 90 °C. Unexpectedly, the immobilized GOD on GNRs still retains about 39.3% activity after the same treatment. This work will be of significance for the biologic enhancement using other kinds of anisotropic nanostructure and suggests a new way of enhancing enzyme thermostability using anisotropic metal nanomaterials. Springer 2009-07-15 /pmc/articles/PMC2894096/ /pubmed/20596428 http://dx.doi.org/10.1007/s11671-009-9385-8 Text en Copyright ©2009 to the authors |
spellingShingle | Nano Express Ma, Zhanfang Ding, Teng Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability |
title | Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability |
title_full | Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability |
title_fullStr | Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability |
title_full_unstemmed | Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability |
title_short | Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability |
title_sort | bioconjugates of glucose oxidase and gold nanorods based on electrostatic interaction with enhanced thermostability |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894096/ https://www.ncbi.nlm.nih.gov/pubmed/20596428 http://dx.doi.org/10.1007/s11671-009-9385-8 |
work_keys_str_mv | AT mazhanfang bioconjugatesofglucoseoxidaseandgoldnanorodsbasedonelectrostaticinteractionwithenhancedthermostability AT dingteng bioconjugatesofglucoseoxidaseandgoldnanorodsbasedonelectrostaticinteractionwithenhancedthermostability |