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A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells

Micro-structures that mimic the extracellular substratum promote cell growth and differentiation, while the cellular reaction to a nanostructure is poorly defined. To evaluate the cellular response to a nanoscaled surface, NIH 3T3 cells were grown on nanodot arrays with dot diameters ranging from 10...

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Autores principales: Pan, Hsu-An, Hung, Yao-Ching, Su, Chia-Wei, Tai, Shih-Ming, Chen, Chiun-Hsun, Ko, Fu-Hsiang, Steve Huang, G
Formato: Texto
Lenguaje:English
Publicado: Springer 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894250/
https://www.ncbi.nlm.nih.gov/pubmed/20596320
http://dx.doi.org/10.1007/s11671-009-9333-7
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author Pan, Hsu-An
Hung, Yao-Ching
Su, Chia-Wei
Tai, Shih-Ming
Chen, Chiun-Hsun
Ko, Fu-Hsiang
Steve Huang, G
author_facet Pan, Hsu-An
Hung, Yao-Ching
Su, Chia-Wei
Tai, Shih-Ming
Chen, Chiun-Hsun
Ko, Fu-Hsiang
Steve Huang, G
author_sort Pan, Hsu-An
collection PubMed
description Micro-structures that mimic the extracellular substratum promote cell growth and differentiation, while the cellular reaction to a nanostructure is poorly defined. To evaluate the cellular response to a nanoscaled surface, NIH 3T3 cells were grown on nanodot arrays with dot diameters ranging from 10 to 200 nm. The nanodot arrays were fabricated by AAO processing on TaN-coated wafers. A thin layer of platinum, 5 nm in thickness, was sputtered onto the structure to improve biocompatibility. The cells grew normally on the 10-nm array and on flat surfaces. However, 50-nm, 100-nm, and 200-nm nanodot arrays induced apoptosis-like events. Abnormality was triggered after as few as 24 h of incubation on a 200-nm dot array. For cells grown on the 50-nm array, the abnormality started after 72 h of incubation. The number of filopodia extended from the cell bodies was lower for the abnormal cells. Immunostaining using antibodies against vinculin and actin filament was performed. Both the number of focal adhesions and the amount of cytoskeleton were decreased in cells grown on the 100-nm and 200-nm arrays. Pre-coatings of fibronectin (FN) or type I collagen promoted cellular anchorage and prevented the nanotopography-induced programed cell death. In summary, nanotopography, in the form of nanodot arrays, induced an apoptosis-like abnormality for cultured NIH 3T3 cells. The occurrence of the abnormality was mediated by the formation of focal adhesions.
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spelling pubmed-28942502010-06-30 A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells Pan, Hsu-An Hung, Yao-Ching Su, Chia-Wei Tai, Shih-Ming Chen, Chiun-Hsun Ko, Fu-Hsiang Steve Huang, G Nanoscale Res Lett Nano Express Micro-structures that mimic the extracellular substratum promote cell growth and differentiation, while the cellular reaction to a nanostructure is poorly defined. To evaluate the cellular response to a nanoscaled surface, NIH 3T3 cells were grown on nanodot arrays with dot diameters ranging from 10 to 200 nm. The nanodot arrays were fabricated by AAO processing on TaN-coated wafers. A thin layer of platinum, 5 nm in thickness, was sputtered onto the structure to improve biocompatibility. The cells grew normally on the 10-nm array and on flat surfaces. However, 50-nm, 100-nm, and 200-nm nanodot arrays induced apoptosis-like events. Abnormality was triggered after as few as 24 h of incubation on a 200-nm dot array. For cells grown on the 50-nm array, the abnormality started after 72 h of incubation. The number of filopodia extended from the cell bodies was lower for the abnormal cells. Immunostaining using antibodies against vinculin and actin filament was performed. Both the number of focal adhesions and the amount of cytoskeleton were decreased in cells grown on the 100-nm and 200-nm arrays. Pre-coatings of fibronectin (FN) or type I collagen promoted cellular anchorage and prevented the nanotopography-induced programed cell death. In summary, nanotopography, in the form of nanodot arrays, induced an apoptosis-like abnormality for cultured NIH 3T3 cells. The occurrence of the abnormality was mediated by the formation of focal adhesions. Springer 2009-05-19 /pmc/articles/PMC2894250/ /pubmed/20596320 http://dx.doi.org/10.1007/s11671-009-9333-7 Text en Copyright ©2009 to the authors
spellingShingle Nano Express
Pan, Hsu-An
Hung, Yao-Ching
Su, Chia-Wei
Tai, Shih-Ming
Chen, Chiun-Hsun
Ko, Fu-Hsiang
Steve Huang, G
A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells
title A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells
title_full A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells
title_fullStr A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells
title_full_unstemmed A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells
title_short A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells
title_sort nanodot array modulates cell adhesion and induces an apoptosis-like abnormality in nih-3t3 cells
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894250/
https://www.ncbi.nlm.nih.gov/pubmed/20596320
http://dx.doi.org/10.1007/s11671-009-9333-7
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