One-Pot Green Synthesis and Bioapplication ofl-Arginine-Capped Superparamagnetic Fe(3)O(4) Nanoparticles

Water-solublel-arginine-capped Fe(3)O(4) nanoparticles were synthesized using a one-pot and green method. Nontoxic, renewable and inexpensive reagents including FeCl(3),l-arginine, glycerol and water were chosen as raw materials. Fe(3)O(4) nanoparticles show different dispersive states in acidic and alkaline solutions for the two distinct forms of surface bindingl-arginine. Powder X-ray diffraction and X-ray photoelectron spectroscopy were used to identify the structure of Fe(3)O(4) nanocrystals. The products behave like superparamagnetism at room temperature with saturation magnetization of 49.9 emu g(−1) and negligible remanence or coercivity. In the presence of 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, the anti-chloramphenicol monoclonal antibodies were connected to thel-arginine-capped magnetite nanoparticles. The as-prepared conjugates could be used in immunomagnetic assay. (See supplementary material 1)