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ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters

Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy of fixed cells showed that ParB mutants lacking the C-terminal domain or HTH motif formed multiple, less intense foci scattered irregularly, in contrast to the...

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Autores principales: Bartosik, A. A., Mierzejewska, J., Thomas, C. M., Jagura-Burdzy, G.
Formato: Texto
Lenguaje:English
Publicado: Microbiology Society 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895232/
https://www.ncbi.nlm.nih.gov/pubmed/19332810
http://dx.doi.org/10.1099/mic.0.024661-0
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author Bartosik, A. A.
Mierzejewska, J.
Thomas, C. M.
Jagura-Burdzy, G.
author_facet Bartosik, A. A.
Mierzejewska, J.
Thomas, C. M.
Jagura-Burdzy, G.
author_sort Bartosik, A. A.
collection PubMed
description Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy of fixed cells showed that ParB mutants lacking the C-terminal domain or HTH motif formed multiple, less intense foci scattered irregularly, in contrast to the one to four ParB foci per cell symmetrically distributed in wild-type P. aeruginosa. All parB mutations affected both bacterial growth and swarming and swimming motilities, and increased the production of anucleate cells. Similar effects were observed after inactivation of parA of P. aeruginosa. As complete loss of ParA destabilized its partner ParB it was unclear deficiency of which protein is responsible for the mutant phenotypes. Analysis of four parB mutants showed that complete loss of ParB destabilized ParA whereas three mutants that retained the N-terminal 90 aa of ParB did not. As all four parB mutants demonstrate the same defects it can be concluded that either ParB, or ParA and ParB in combination, plays an important role in nucleoid distribution, growth and motility in P. aeruginosa.
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spelling pubmed-28952322010-07-06 ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters Bartosik, A. A. Mierzejewska, J. Thomas, C. M. Jagura-Burdzy, G. Microbiology (Reading) Cell and Molecular Biology of Microbes Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy of fixed cells showed that ParB mutants lacking the C-terminal domain or HTH motif formed multiple, less intense foci scattered irregularly, in contrast to the one to four ParB foci per cell symmetrically distributed in wild-type P. aeruginosa. All parB mutations affected both bacterial growth and swarming and swimming motilities, and increased the production of anucleate cells. Similar effects were observed after inactivation of parA of P. aeruginosa. As complete loss of ParA destabilized its partner ParB it was unclear deficiency of which protein is responsible for the mutant phenotypes. Analysis of four parB mutants showed that complete loss of ParB destabilized ParA whereas three mutants that retained the N-terminal 90 aa of ParB did not. As all four parB mutants demonstrate the same defects it can be concluded that either ParB, or ParA and ParB in combination, plays an important role in nucleoid distribution, growth and motility in P. aeruginosa. Microbiology Society 2009-04 /pmc/articles/PMC2895232/ /pubmed/19332810 http://dx.doi.org/10.1099/mic.0.024661-0 Text en Copyright © 2009, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cell and Molecular Biology of Microbes
Bartosik, A. A.
Mierzejewska, J.
Thomas, C. M.
Jagura-Burdzy, G.
ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
title ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
title_full ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
title_fullStr ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
title_full_unstemmed ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
title_short ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
title_sort parb deficiency in pseudomonas aeruginosa destabilizes the partner protein para and affects a variety of physiological parameters
topic Cell and Molecular Biology of Microbes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895232/
https://www.ncbi.nlm.nih.gov/pubmed/19332810
http://dx.doi.org/10.1099/mic.0.024661-0
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