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Munc18 and Munc13 regulate early neurite outgrowth
Background information. During development, growth cones of outgrowing neurons express proteins involved in vesicular secretion, such as SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins, Munc13 and Munc18. Vesicles are known to fuse in growth cones prior...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895782/ https://www.ncbi.nlm.nih.gov/pubmed/20497124 http://dx.doi.org/10.1042/BC20100036 |
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author | Broeke, Jurjen H.P. Roelandse, Martijn Luteijn, Maartje J. Boiko, Tatiana Matus, Andrew Toonen, Ruud F. Verhage, Matthijs |
author_facet | Broeke, Jurjen H.P. Roelandse, Martijn Luteijn, Maartje J. Boiko, Tatiana Matus, Andrew Toonen, Ruud F. Verhage, Matthijs |
author_sort | Broeke, Jurjen H.P. |
collection | PubMed |
description | Background information. During development, growth cones of outgrowing neurons express proteins involved in vesicular secretion, such as SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins, Munc13 and Munc18. Vesicles are known to fuse in growth cones prior to synapse formation, which may contribute to outgrowth. Results. We tested this possibility in dissociated cell cultures and organotypic slice cultures of two release-deficient mice (Munc18-1 null and Munc13-1/2 double null). Both types of release-deficient neurons have a decreased outgrowth speed and therefore have a smaller total neurite length during early development [DIV1–4 (day in vitro 1–4)]. In addition, more filopodia per growth cone were observed in Munc18-1 null, but not WT (wild-type) or Munc13-1/2 double null neurons. The smaller total neurite length during early development was no longer observed after synaptogenesis (DIV14–23). Conclusion. These data suggest that the inability of vesicle fusion in the growth cone affects outgrowth during the initial phases when outgrowth speed is high, but not during/after synaptogenesis. Overall, the outgrowth speed is probably not rate-limiting during neuronal network formation, at least in vitro. In addition, Munc18, but not Munc13, regulates growth cone filopodia, potentially via its previously observed effect on filamentous actin. |
format | Text |
id | pubmed-2895782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-28957822010-07-07 Munc18 and Munc13 regulate early neurite outgrowth Broeke, Jurjen H.P. Roelandse, Martijn Luteijn, Maartje J. Boiko, Tatiana Matus, Andrew Toonen, Ruud F. Verhage, Matthijs Biol Cell Research Article Background information. During development, growth cones of outgrowing neurons express proteins involved in vesicular secretion, such as SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins, Munc13 and Munc18. Vesicles are known to fuse in growth cones prior to synapse formation, which may contribute to outgrowth. Results. We tested this possibility in dissociated cell cultures and organotypic slice cultures of two release-deficient mice (Munc18-1 null and Munc13-1/2 double null). Both types of release-deficient neurons have a decreased outgrowth speed and therefore have a smaller total neurite length during early development [DIV1–4 (day in vitro 1–4)]. In addition, more filopodia per growth cone were observed in Munc18-1 null, but not WT (wild-type) or Munc13-1/2 double null neurons. The smaller total neurite length during early development was no longer observed after synaptogenesis (DIV14–23). Conclusion. These data suggest that the inability of vesicle fusion in the growth cone affects outgrowth during the initial phases when outgrowth speed is high, but not during/after synaptogenesis. Overall, the outgrowth speed is probably not rate-limiting during neuronal network formation, at least in vitro. In addition, Munc18, but not Munc13, regulates growth cone filopodia, potentially via its previously observed effect on filamentous actin. Portland Press Ltd. 2010-06-30 /pmc/articles/PMC2895782/ /pubmed/20497124 http://dx.doi.org/10.1042/BC20100036 Text en © 2010 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by-nc/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Broeke, Jurjen H.P. Roelandse, Martijn Luteijn, Maartje J. Boiko, Tatiana Matus, Andrew Toonen, Ruud F. Verhage, Matthijs Munc18 and Munc13 regulate early neurite outgrowth |
title | Munc18 and Munc13 regulate early neurite outgrowth |
title_full | Munc18 and Munc13 regulate early neurite outgrowth |
title_fullStr | Munc18 and Munc13 regulate early neurite outgrowth |
title_full_unstemmed | Munc18 and Munc13 regulate early neurite outgrowth |
title_short | Munc18 and Munc13 regulate early neurite outgrowth |
title_sort | munc18 and munc13 regulate early neurite outgrowth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895782/ https://www.ncbi.nlm.nih.gov/pubmed/20497124 http://dx.doi.org/10.1042/BC20100036 |
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