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A Genetic Engineering Solution to the “Arginine Conversion Problem” in Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Stable isotope labeling by amino acids in cell culture (SILAC) provides a straightforward tool for quantitation in proteomics. However, one problem associated with SILAC is the in vivo conversion of labeled arginine to other amino acids, typically proline. We found that arginine conversion in the fi...

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Detalles Bibliográficos
Autores principales:	Bicho, Claudia C., de Lima Alves, Flavia, Chen, Zhuo A., Rappsilber, Juri, Sawin, Kenneth E.
Formato:	Texto
Lenguaje:	English
Publicado:	The American Society for Biochemistry and Molecular Biology 2010
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896365/ https://www.ncbi.nlm.nih.gov/pubmed/20460254 http://dx.doi.org/10.1074/mcp.M110.000208

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