The crystal structure of unmodified tRNA(Phe) from Escherichia coli

Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report the crystal structure of unmodified tRNA(Phe) from Escherichia coli at a resolution of 3 Å. We sho...

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Detalles Bibliográficos
Autores principales: Byrne, Robert T., Konevega, Andrey L., Rodnina, Marina V., Antson, Alfred A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896525/
https://www.ncbi.nlm.nih.gov/pubmed/20203084
http://dx.doi.org/10.1093/nar/gkq133
Descripción
Sumario:Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report the crystal structure of unmodified tRNA(Phe) from Escherichia coli at a resolution of 3 Å. We show that in the absence of modifications the overall fold of the tRNA is essentially the same as that of mature tRNA. However, there are a number of significant structural differences, such as rearrangements in a triplet base pair and a widened angle between the acceptor and anticodon stems. Contrary to previous observations, the anticodon adopts the same conformation as seen in mature tRNA.

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