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Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”

A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White...

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Detalles Bibliográficos
Autores principales: Sun, Jian, Wang, Hexiang, Ng, Tzi Bun
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896657/
https://www.ncbi.nlm.nih.gov/pubmed/20617140
http://dx.doi.org/10.1155/2010/219793
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author Sun, Jian
Wang, Hexiang
Ng, Tzi Bun
author_facet Sun, Jian
Wang, Hexiang
Ng, Tzi Bun
author_sort Sun, Jian
collection PubMed
description A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16 kDa and reduced the activity of trypsin with an IC(50) value of about 0.6 μM. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-(3)H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC(50) value of 28.8 μM and 21.5 μM, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100 μM.
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spelling pubmed-28966572010-07-08 Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean” Sun, Jian Wang, Hexiang Ng, Tzi Bun J Biomed Biotechnol Research Article A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16 kDa and reduced the activity of trypsin with an IC(50) value of about 0.6 μM. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-(3)H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC(50) value of 28.8 μM and 21.5 μM, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100 μM. Hindawi Publishing Corporation 2010 2010-06-14 /pmc/articles/PMC2896657/ /pubmed/20617140 http://dx.doi.org/10.1155/2010/219793 Text en Copyright © 2010 Jian Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sun, Jian
Wang, Hexiang
Ng, Tzi Bun
Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
title Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
title_full Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
title_fullStr Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
title_full_unstemmed Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
title_short Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
title_sort trypsin isoinhibitors with antiproliferative activity toward leukemia cells from phaseolus vulgaris cv “white cloud bean”
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896657/
https://www.ncbi.nlm.nih.gov/pubmed/20617140
http://dx.doi.org/10.1155/2010/219793
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