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High Throughput T Epitope Mapping and Vaccine Development
Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libr...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896667/ https://www.ncbi.nlm.nih.gov/pubmed/20617148 http://dx.doi.org/10.1155/2010/325720 |
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author | Li Pira, Giuseppina Ivaldi, Federico Moretti, Paolo Manca, Fabrizio |
author_facet | Li Pira, Giuseppina Ivaldi, Federico Moretti, Paolo Manca, Fabrizio |
author_sort | Li Pira, Giuseppina |
collection | PubMed |
description | Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libraries to monitor specific cellular immunity in protected individuals, patients and vaccinees. Nevertheless, epitope mapping is a challenging task. In fact, large panels of overlapping peptides need to be tested with lymphocytes to identify the sequences that induce a T-cell response. Since numerous peptide panels from antigenic proteins are to be screened, lymphocytes available from human subjects are a limiting factor. To overcome this limitation, high throughput (HTP) approaches based on miniaturization and automation of T-cell assays are needed. Here we consider the most recent applications of the HTP approach to T epitope mapping. The alternative or complementary use of in silico prediction and experimental epitope definition is discussed in the context of the recent literature. The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost. |
format | Text |
id | pubmed-2896667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-28966672010-07-08 High Throughput T Epitope Mapping and Vaccine Development Li Pira, Giuseppina Ivaldi, Federico Moretti, Paolo Manca, Fabrizio J Biomed Biotechnol Review Article Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libraries to monitor specific cellular immunity in protected individuals, patients and vaccinees. Nevertheless, epitope mapping is a challenging task. In fact, large panels of overlapping peptides need to be tested with lymphocytes to identify the sequences that induce a T-cell response. Since numerous peptide panels from antigenic proteins are to be screened, lymphocytes available from human subjects are a limiting factor. To overcome this limitation, high throughput (HTP) approaches based on miniaturization and automation of T-cell assays are needed. Here we consider the most recent applications of the HTP approach to T epitope mapping. The alternative or complementary use of in silico prediction and experimental epitope definition is discussed in the context of the recent literature. The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost. Hindawi Publishing Corporation 2010 2010-06-15 /pmc/articles/PMC2896667/ /pubmed/20617148 http://dx.doi.org/10.1155/2010/325720 Text en Copyright © 2010 Giuseppina Li Pira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Li Pira, Giuseppina Ivaldi, Federico Moretti, Paolo Manca, Fabrizio High Throughput T Epitope Mapping and Vaccine Development |
title | High Throughput T Epitope Mapping and Vaccine Development |
title_full | High Throughput T Epitope Mapping and Vaccine Development |
title_fullStr | High Throughput T Epitope Mapping and Vaccine Development |
title_full_unstemmed | High Throughput T Epitope Mapping and Vaccine Development |
title_short | High Throughput T Epitope Mapping and Vaccine Development |
title_sort | high throughput t epitope mapping and vaccine development |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896667/ https://www.ncbi.nlm.nih.gov/pubmed/20617148 http://dx.doi.org/10.1155/2010/325720 |
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