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Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro

Nigella sativa L. (NS) has been used for medicinal purposes since ancient times. This study aimed to investigate the cytotoxicity of NS dry methanolic extract on cultured cortical neurons and its influence on neurotransmitter release, as well as the presence of excitatory (glutamate and aspartate) a...

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Autores principales: El-Naggar, Tarek, Gómez-Serranillos, María Pilar, Palomino, Olga María, Arce, Carmen, Carretero, María Emilia
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896676/
https://www.ncbi.nlm.nih.gov/pubmed/20625485
http://dx.doi.org/10.1155/2010/398312
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author El-Naggar, Tarek
Gómez-Serranillos, María Pilar
Palomino, Olga María
Arce, Carmen
Carretero, María Emilia
author_facet El-Naggar, Tarek
Gómez-Serranillos, María Pilar
Palomino, Olga María
Arce, Carmen
Carretero, María Emilia
author_sort El-Naggar, Tarek
collection PubMed
description Nigella sativa L. (NS) has been used for medicinal purposes since ancient times. This study aimed to investigate the cytotoxicity of NS dry methanolic extract on cultured cortical neurons and its influence on neurotransmitter release, as well as the presence of excitatory (glutamate and aspartate) and inhibitory amino acids (gamma-aminobutyric acid—GABA—and glycine) in NS extract. Cultured rat cortical neurons were exposed to different times and concentrations of NS dry methanolic extract and cell viability was then determined by a quantitative colorimetric method. NS did not induce any toxicity. The secretion of different amino acids was studied in primary cultured cortical neurons by high-performance liquid chromatography (HPLC) using a derivation before injection with dansyl chloride. NS modulated amino acid release in cultured neurons; GABA was significantly increased whereas secretion of glutamate, aspartate, and glycine were decreased. The in vitro findings support the hypothesis that the sedative and depressive effects of NS observed in vivo could be based on changes of inhibitory/excitatory amino acids levels.
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spelling pubmed-28966762010-07-12 Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro El-Naggar, Tarek Gómez-Serranillos, María Pilar Palomino, Olga María Arce, Carmen Carretero, María Emilia J Biomed Biotechnol Research Article Nigella sativa L. (NS) has been used for medicinal purposes since ancient times. This study aimed to investigate the cytotoxicity of NS dry methanolic extract on cultured cortical neurons and its influence on neurotransmitter release, as well as the presence of excitatory (glutamate and aspartate) and inhibitory amino acids (gamma-aminobutyric acid—GABA—and glycine) in NS extract. Cultured rat cortical neurons were exposed to different times and concentrations of NS dry methanolic extract and cell viability was then determined by a quantitative colorimetric method. NS did not induce any toxicity. The secretion of different amino acids was studied in primary cultured cortical neurons by high-performance liquid chromatography (HPLC) using a derivation before injection with dansyl chloride. NS modulated amino acid release in cultured neurons; GABA was significantly increased whereas secretion of glutamate, aspartate, and glycine were decreased. The in vitro findings support the hypothesis that the sedative and depressive effects of NS observed in vivo could be based on changes of inhibitory/excitatory amino acids levels. Hindawi Publishing Corporation 2010 2010-06-16 /pmc/articles/PMC2896676/ /pubmed/20625485 http://dx.doi.org/10.1155/2010/398312 Text en Copyright © 2010 Tarek El-Naggar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
El-Naggar, Tarek
Gómez-Serranillos, María Pilar
Palomino, Olga María
Arce, Carmen
Carretero, María Emilia
Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
title Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
title_full Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
title_fullStr Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
title_full_unstemmed Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
title_short Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
title_sort nigella sativa l. seed extract modulates the neurotransmitter amino acids release in cultured neurons in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896676/
https://www.ncbi.nlm.nih.gov/pubmed/20625485
http://dx.doi.org/10.1155/2010/398312
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