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Optimization of Formaldehyde Cross-Linking for Protein Interaction Analysis of Non-Tagged Integrin β1

Formaldehyde cross-linking of protein complexes combined with immunoprecipitation and mass spectrometry analysis is a promising technique for analysing protein-protein interactions, including those of transient nature. Here we used integrin β1 as a model to describe the application of formaldehyde c...

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Detalles Bibliográficos
Autores principales: Klockenbusch, Cordula, Kast, Juergen
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896913/
https://www.ncbi.nlm.nih.gov/pubmed/20634879
http://dx.doi.org/10.1155/2010/927585