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Expression and Purification of Z Protein from Junín Virus

Arenaviridae comprises 23 recognized virus species with a bipartite ssRNA genome and an ambisense coding strategy. The virions are enveloped and include nonequimolar amounts of each genomic RNA species, designated L and S, coding for four ORFs (N, GPC, L, and Z). The arenavirus Junín (JUNV) is the e...

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Autores principales: Goñi, S. E., Borio, C. S., Romano, F. B., Rota, R. P., Pilloff, M. G., Iserte, J. A., Tortorici, M. A., Stephan, B. I., Bilen, M. F., Ghiringhelli, P. D., Lozano, M. E.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896916/
https://www.ncbi.nlm.nih.gov/pubmed/20652066
http://dx.doi.org/10.1155/2010/970491
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author Goñi, S. E.
Borio, C. S.
Romano, F. B.
Rota, R. P.
Pilloff, M. G.
Iserte, J. A.
Tortorici, M. A.
Stephan, B. I.
Bilen, M. F.
Ghiringhelli, P. D.
Lozano, M. E.
author_facet Goñi, S. E.
Borio, C. S.
Romano, F. B.
Rota, R. P.
Pilloff, M. G.
Iserte, J. A.
Tortorici, M. A.
Stephan, B. I.
Bilen, M. F.
Ghiringhelli, P. D.
Lozano, M. E.
author_sort Goñi, S. E.
collection PubMed
description Arenaviridae comprises 23 recognized virus species with a bipartite ssRNA genome and an ambisense coding strategy. The virions are enveloped and include nonequimolar amounts of each genomic RNA species, designated L and S, coding for four ORFs (N, GPC, L, and Z). The arenavirus Junín (JUNV) is the etiological agent of Argentine Hemorrhagic Fever, an acute disease with high mortality rate. It has been proposed that Z is the functional counterpart of the matrix proteins found in other negative-stranded enveloped RNA viruses. Here we report the optimized expression of a synthetic gene of Z protein, using three expression systems (two bacterial and a baculoviral one). One of these recombinant proteins was used to generate antibodies. A bioinformatic analysis was made where Z was subdivided into three domains. The data presented contributes methodologies for Z recombinant production and provides the basis for the development of new experiments to test its function.
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spelling pubmed-28969162010-07-22 Expression and Purification of Z Protein from Junín Virus Goñi, S. E. Borio, C. S. Romano, F. B. Rota, R. P. Pilloff, M. G. Iserte, J. A. Tortorici, M. A. Stephan, B. I. Bilen, M. F. Ghiringhelli, P. D. Lozano, M. E. J Biomed Biotechnol Research Article Arenaviridae comprises 23 recognized virus species with a bipartite ssRNA genome and an ambisense coding strategy. The virions are enveloped and include nonequimolar amounts of each genomic RNA species, designated L and S, coding for four ORFs (N, GPC, L, and Z). The arenavirus Junín (JUNV) is the etiological agent of Argentine Hemorrhagic Fever, an acute disease with high mortality rate. It has been proposed that Z is the functional counterpart of the matrix proteins found in other negative-stranded enveloped RNA viruses. Here we report the optimized expression of a synthetic gene of Z protein, using three expression systems (two bacterial and a baculoviral one). One of these recombinant proteins was used to generate antibodies. A bioinformatic analysis was made where Z was subdivided into three domains. The data presented contributes methodologies for Z recombinant production and provides the basis for the development of new experiments to test its function. Hindawi Publishing Corporation 2010 2010-06-29 /pmc/articles/PMC2896916/ /pubmed/20652066 http://dx.doi.org/10.1155/2010/970491 Text en Copyright © 2010 S. E. Goñi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Goñi, S. E.
Borio, C. S.
Romano, F. B.
Rota, R. P.
Pilloff, M. G.
Iserte, J. A.
Tortorici, M. A.
Stephan, B. I.
Bilen, M. F.
Ghiringhelli, P. D.
Lozano, M. E.
Expression and Purification of Z Protein from Junín Virus
title Expression and Purification of Z Protein from Junín Virus
title_full Expression and Purification of Z Protein from Junín Virus
title_fullStr Expression and Purification of Z Protein from Junín Virus
title_full_unstemmed Expression and Purification of Z Protein from Junín Virus
title_short Expression and Purification of Z Protein from Junín Virus
title_sort expression and purification of z protein from junín virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896916/
https://www.ncbi.nlm.nih.gov/pubmed/20652066
http://dx.doi.org/10.1155/2010/970491
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