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Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum

BACKGROUND: To maintain pluripotency of human embryonic stem (huES) cells in feeder-free culture it has been necessary to provide a Matrigel substratum, which is a complex of poorly defined extracellular matrices and growth factors derived from mouse Engelbreth-Holm-Swarm sarcoma cells. Culture of s...

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Detalles Bibliográficos
Autores principales:	Nagaoka, Masato, Si-Tayeb, Karim, Akaike, Toshihiro, Duncan, Stephen A
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896937/ https://www.ncbi.nlm.nih.gov/pubmed/20525219 http://dx.doi.org/10.1186/1471-213X-10-60

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