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Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells
Endogenous estrogens have dramatic and differential effects on classical endocrine organ and proliferation. Xenoestrogens are environmental estrogens that have endocrine impact, acting as both estrogen agonists and antagonists, but whose effects are not well characterized. In this investigation we s...
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Formato: | Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898035/ https://www.ncbi.nlm.nih.gov/pubmed/20623010 http://dx.doi.org/10.3390/ijerph7052045 |
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author | Ndebele, Kenneth Graham, Barbara Tchounwou, Paul B. |
author_facet | Ndebele, Kenneth Graham, Barbara Tchounwou, Paul B. |
author_sort | Ndebele, Kenneth |
collection | PubMed |
description | Endogenous estrogens have dramatic and differential effects on classical endocrine organ and proliferation. Xenoestrogens are environmental estrogens that have endocrine impact, acting as both estrogen agonists and antagonists, but whose effects are not well characterized. In this investigation we sought to delineate effects of xenoestrogens. Using human cervical cancer cells (HeLa cells) as a model, the effects of representative xenoestrogens (Coumestrol-a phytoestrogen, tetrachlorodioxin (TCDD)-a herbicide and DDT-a pesticide) on proliferation, cell cycle, and apoptosis were examined. These xenoestrogens and estrogen inhibited the proliferation of Hela cells in a dose dependent manner from 20 to 120 nM suggesting, that 17-β-estrtadiol and xenoestrogens induced cytotoxic effects. Coumestrol produced accumulation of HeLa cells in G2/M phase, and subsequently induced apoptosis. Similar effects were observed in estrogen treated cells. These changes were associated with suppressed bcl-2 protein and augmented Cyclins A and D proteins. DDT and TCDD exposure did not induce apoptosis. These preliminary data taken together, suggest that xenoestrogens have direct, compound-specific effects on HeLa cells. This study further enhances our understanding of environmental modulation of cervical cancer. |
format | Text |
id | pubmed-2898035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-28980352010-07-09 Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells Ndebele, Kenneth Graham, Barbara Tchounwou, Paul B. Int J Environ Res Public Health Article Endogenous estrogens have dramatic and differential effects on classical endocrine organ and proliferation. Xenoestrogens are environmental estrogens that have endocrine impact, acting as both estrogen agonists and antagonists, but whose effects are not well characterized. In this investigation we sought to delineate effects of xenoestrogens. Using human cervical cancer cells (HeLa cells) as a model, the effects of representative xenoestrogens (Coumestrol-a phytoestrogen, tetrachlorodioxin (TCDD)-a herbicide and DDT-a pesticide) on proliferation, cell cycle, and apoptosis were examined. These xenoestrogens and estrogen inhibited the proliferation of Hela cells in a dose dependent manner from 20 to 120 nM suggesting, that 17-β-estrtadiol and xenoestrogens induced cytotoxic effects. Coumestrol produced accumulation of HeLa cells in G2/M phase, and subsequently induced apoptosis. Similar effects were observed in estrogen treated cells. These changes were associated with suppressed bcl-2 protein and augmented Cyclins A and D proteins. DDT and TCDD exposure did not induce apoptosis. These preliminary data taken together, suggest that xenoestrogens have direct, compound-specific effects on HeLa cells. This study further enhances our understanding of environmental modulation of cervical cancer. Molecular Diversity Preservation International (MDPI) 2010-05-04 2010-05 /pmc/articles/PMC2898035/ /pubmed/20623010 http://dx.doi.org/10.3390/ijerph7052045 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Ndebele, Kenneth Graham, Barbara Tchounwou, Paul B. Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells |
title | Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells |
title_full | Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells |
title_fullStr | Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells |
title_full_unstemmed | Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells |
title_short | Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells |
title_sort | estrogenic activity of coumestrol, ddt, and tcdd in human cervical cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898035/ https://www.ncbi.nlm.nih.gov/pubmed/20623010 http://dx.doi.org/10.3390/ijerph7052045 |
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