Computational challenges in the analysis of ancient DNA

High-throughput sequencing technologies have opened up a new avenue for studying extinct organisms. Here we identify and quantify biases introduced by particular characteristics of ancient DNA samples. These analyses demonstrate the importance of closely related genomic sequence for correctly identi...

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Detalles Bibliográficos
Autores principales: Prüfer, Kay, Stenzel, Udo, Hofreiter, Michael, Pääbo, Svante, Kelso, Janet, Green, Richard E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898072/
https://www.ncbi.nlm.nih.gov/pubmed/20441577
http://dx.doi.org/10.1186/gb-2010-11-5-r47
Descripción
Sumario:High-throughput sequencing technologies have opened up a new avenue for studying extinct organisms. Here we identify and quantify biases introduced by particular characteristics of ancient DNA samples. These analyses demonstrate the importance of closely related genomic sequence for correctly identifying and classifying bona fide endogenous DNA fragments. We show that more accurate genome divergence estimates from ancient DNA sequence can be attained using at least two outgroup genomes and appropriate filtering.

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