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Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis
Shoot branching and growth are controlled by phytohormones such as auxin and other components in Arabidopsis. We identified a mutant (igi1) showing decreased height and bunchy branching patterns. The phenotypes reverted to the wild type in response to RNA interference with the IGI1 gene. Histochemic...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898107/ https://www.ncbi.nlm.nih.gov/pubmed/20473553 http://dx.doi.org/10.1007/s11103-010-9645-0 |
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author | Hwang, Indeok Kim, Soo Young Kim, Cheol Soo Park, Yoonkyung Tripathi, Giri Raj Kim, Seong-Ki Cheong, Hyeonsook |
author_facet | Hwang, Indeok Kim, Soo Young Kim, Cheol Soo Park, Yoonkyung Tripathi, Giri Raj Kim, Seong-Ki Cheong, Hyeonsook |
author_sort | Hwang, Indeok |
collection | PubMed |
description | Shoot branching and growth are controlled by phytohormones such as auxin and other components in Arabidopsis. We identified a mutant (igi1) showing decreased height and bunchy branching patterns. The phenotypes reverted to the wild type in response to RNA interference with the IGI1 gene. Histochemical analysis by GUS assay revealed tissue-specific gene expression in the anther and showed that the expression levels of the IGI1 gene in apical parts, including flowers, were higher than in other parts of the plants. The auxin biosynthesis component gene, CYP79B2, was up-regulated in igi1 mutants and the IGI1 gene was down-regulated by IAA treatment. These results indicated that there is an interplay regulation between IGI1 and phytohormone auxin. Moreover, the expression of the auxin-related shoot branching regulation genes, MAX3 and MAX4, was down-regulated in igi1 mutants. Taken together, these results indicate that the overexpression of the IGI1 influenced MAX pathway in the shoot branching regulation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-010-9645-0) contains supplementary material, which is available to authorized users. |
format | Text |
id | pubmed-2898107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-28981072010-07-29 Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis Hwang, Indeok Kim, Soo Young Kim, Cheol Soo Park, Yoonkyung Tripathi, Giri Raj Kim, Seong-Ki Cheong, Hyeonsook Plant Mol Biol Article Shoot branching and growth are controlled by phytohormones such as auxin and other components in Arabidopsis. We identified a mutant (igi1) showing decreased height and bunchy branching patterns. The phenotypes reverted to the wild type in response to RNA interference with the IGI1 gene. Histochemical analysis by GUS assay revealed tissue-specific gene expression in the anther and showed that the expression levels of the IGI1 gene in apical parts, including flowers, were higher than in other parts of the plants. The auxin biosynthesis component gene, CYP79B2, was up-regulated in igi1 mutants and the IGI1 gene was down-regulated by IAA treatment. These results indicated that there is an interplay regulation between IGI1 and phytohormone auxin. Moreover, the expression of the auxin-related shoot branching regulation genes, MAX3 and MAX4, was down-regulated in igi1 mutants. Taken together, these results indicate that the overexpression of the IGI1 influenced MAX pathway in the shoot branching regulation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-010-9645-0) contains supplementary material, which is available to authorized users. Springer Netherlands 2010-05-15 2010 /pmc/articles/PMC2898107/ /pubmed/20473553 http://dx.doi.org/10.1007/s11103-010-9645-0 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Hwang, Indeok Kim, Soo Young Kim, Cheol Soo Park, Yoonkyung Tripathi, Giri Raj Kim, Seong-Ki Cheong, Hyeonsook Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis |
title | Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis |
title_full | Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis |
title_fullStr | Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis |
title_full_unstemmed | Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis |
title_short | Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis |
title_sort | over-expression of the igi1 leading to altered shoot-branching development related to max pathway in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898107/ https://www.ncbi.nlm.nih.gov/pubmed/20473553 http://dx.doi.org/10.1007/s11103-010-9645-0 |
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