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Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution

We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm(2). By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line...

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Detalles Bibliográficos
Autores principales: Bishara, Waheb, Su, Ting-Wei, Coskun, Ahmet F., Ozcan, Aydogan
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898729/
https://www.ncbi.nlm.nih.gov/pubmed/20588977
http://dx.doi.org/10.1364/OE.18.011181
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author Bishara, Waheb
Su, Ting-Wei
Coskun, Ahmet F.
Ozcan, Aydogan
author_facet Bishara, Waheb
Su, Ting-Wei
Coskun, Ahmet F.
Ozcan, Aydogan
author_sort Bishara, Waheb
collection PubMed
description We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm(2). By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm(2)) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans.
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spelling pubmed-28987292010-07-07 Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution Bishara, Waheb Su, Ting-Wei Coskun, Ahmet F. Ozcan, Aydogan Opt Express Research-Article We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm(2). By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm(2)) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans. Optical Society of America 2010-05-12 /pmc/articles/PMC2898729/ /pubmed/20588977 http://dx.doi.org/10.1364/OE.18.011181 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Bishara, Waheb
Su, Ting-Wei
Coskun, Ahmet F.
Ozcan, Aydogan
Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
title Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
title_full Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
title_fullStr Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
title_full_unstemmed Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
title_short Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
title_sort lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898729/
https://www.ncbi.nlm.nih.gov/pubmed/20588977
http://dx.doi.org/10.1364/OE.18.011181
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