Cargando…
Morphine induces preconditioning via activation of mitochondrial K(Ca) channels
PURPOSE: Mitochondrial calcium sensitive potassium (mK(Ca)) channels are involved in cardioprotection induced by ischemic preconditioning. In the present study we investigated whether morphine-induced preconditioning also involves activation of mK(Ca) channels. METHODS: Isolated rat hearts (six grou...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2899019/ https://www.ncbi.nlm.nih.gov/pubmed/20461490 http://dx.doi.org/10.1007/s12630-010-9325-1 |
_version_ | 1782183547991228416 |
---|---|
author | Fräßdorf, Jan Huhn, Ragnar Niersmann, Corinna Weber, Nina C. Schlack, Wolfgang Preckel, Benedikt Hollmann, Markus W. |
author_facet | Fräßdorf, Jan Huhn, Ragnar Niersmann, Corinna Weber, Nina C. Schlack, Wolfgang Preckel, Benedikt Hollmann, Markus W. |
author_sort | Fräßdorf, Jan |
collection | PubMed |
description | PURPOSE: Mitochondrial calcium sensitive potassium (mK(Ca)) channels are involved in cardioprotection induced by ischemic preconditioning. In the present study we investigated whether morphine-induced preconditioning also involves activation of mK(Ca) channels. METHODS: Isolated rat hearts (six groups; each n = 8) underwent global ischemia for 30 min followed by a 60-min reperfusion. Control animals were not further treated. Morphine preconditioning (MPC) was initiated by two five-minute cycles of morphine 1 μM infusion with one five-minute washout and one final ten-minute washout period before ischemia. The mK(Ca) blocker, paxilline 1 μM, was administered, with and without morphine administration (MPC + Pax and Pax). As a positive control, we added an ischemic preconditioning group (IPC) alone and combined with paxilline (IPC + Pax). At the end of reperfusion, infarct sizes were determined by triphenyltetrazoliumchloride staining. RESULTS: Infarct size was (mean ± SD) 45 ± 9% of the area at risk in the Control group. The infarct size was less in the morphine or ischemic preconditioning groups (MPC: 23 ± 8%, IPC: 20 ± 5%; each P < 0.05 vs Control). Infarct size reduction was abolished by paxilline (MPC + Pax: 37 ± 7%, P < 0.05 vs MPC and IPC + Pax: 36 ± 6%, P < 0.05 vs IPC), whereas paxilline alone had no effect (Pax: 46 ± 7%, not significantly different from Control). CONCLUSION: Cardioprotection by morphine-induced preconditioning is mediated by activation of mK(Ca) channels. |
format | Text |
id | pubmed-2899019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-28990192010-07-29 Morphine induces preconditioning via activation of mitochondrial K(Ca) channels Fräßdorf, Jan Huhn, Ragnar Niersmann, Corinna Weber, Nina C. Schlack, Wolfgang Preckel, Benedikt Hollmann, Markus W. Can J Anaesth Reports of Original Investigations PURPOSE: Mitochondrial calcium sensitive potassium (mK(Ca)) channels are involved in cardioprotection induced by ischemic preconditioning. In the present study we investigated whether morphine-induced preconditioning also involves activation of mK(Ca) channels. METHODS: Isolated rat hearts (six groups; each n = 8) underwent global ischemia for 30 min followed by a 60-min reperfusion. Control animals were not further treated. Morphine preconditioning (MPC) was initiated by two five-minute cycles of morphine 1 μM infusion with one five-minute washout and one final ten-minute washout period before ischemia. The mK(Ca) blocker, paxilline 1 μM, was administered, with and without morphine administration (MPC + Pax and Pax). As a positive control, we added an ischemic preconditioning group (IPC) alone and combined with paxilline (IPC + Pax). At the end of reperfusion, infarct sizes were determined by triphenyltetrazoliumchloride staining. RESULTS: Infarct size was (mean ± SD) 45 ± 9% of the area at risk in the Control group. The infarct size was less in the morphine or ischemic preconditioning groups (MPC: 23 ± 8%, IPC: 20 ± 5%; each P < 0.05 vs Control). Infarct size reduction was abolished by paxilline (MPC + Pax: 37 ± 7%, P < 0.05 vs MPC and IPC + Pax: 36 ± 6%, P < 0.05 vs IPC), whereas paxilline alone had no effect (Pax: 46 ± 7%, not significantly different from Control). CONCLUSION: Cardioprotection by morphine-induced preconditioning is mediated by activation of mK(Ca) channels. Springer-Verlag 2010-05-12 2010 /pmc/articles/PMC2899019/ /pubmed/20461490 http://dx.doi.org/10.1007/s12630-010-9325-1 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Reports of Original Investigations Fräßdorf, Jan Huhn, Ragnar Niersmann, Corinna Weber, Nina C. Schlack, Wolfgang Preckel, Benedikt Hollmann, Markus W. Morphine induces preconditioning via activation of mitochondrial K(Ca) channels |
title | Morphine induces preconditioning via activation of mitochondrial K(Ca) channels |
title_full | Morphine induces preconditioning via activation of mitochondrial K(Ca) channels |
title_fullStr | Morphine induces preconditioning via activation of mitochondrial K(Ca) channels |
title_full_unstemmed | Morphine induces preconditioning via activation of mitochondrial K(Ca) channels |
title_short | Morphine induces preconditioning via activation of mitochondrial K(Ca) channels |
title_sort | morphine induces preconditioning via activation of mitochondrial k(ca) channels |
topic | Reports of Original Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2899019/ https://www.ncbi.nlm.nih.gov/pubmed/20461490 http://dx.doi.org/10.1007/s12630-010-9325-1 |
work_keys_str_mv | AT fraßdorfjan morphineinducespreconditioningviaactivationofmitochondrialkcachannels AT huhnragnar morphineinducespreconditioningviaactivationofmitochondrialkcachannels AT niersmanncorinna morphineinducespreconditioningviaactivationofmitochondrialkcachannels AT weberninac morphineinducespreconditioningviaactivationofmitochondrialkcachannels AT schlackwolfgang morphineinducespreconditioningviaactivationofmitochondrialkcachannels AT preckelbenedikt morphineinducespreconditioningviaactivationofmitochondrialkcachannels AT hollmannmarkusw morphineinducespreconditioningviaactivationofmitochondrialkcachannels |