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Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method

A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of oleanolic acid and ursolic acid contents in the Ziziphora clinopodioides Lam. with short run time. Chromatographic separation is achieved by using HPLC syste...

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Autores principales: Tian, Shuge, Shi, Yang, Yu, Qian, Upur, Halmurat
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900058/
https://www.ncbi.nlm.nih.gov/pubmed/20668577
http://dx.doi.org/10.4103/0973-1296.62898
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author Tian, Shuge
Shi, Yang
Yu, Qian
Upur, Halmurat
author_facet Tian, Shuge
Shi, Yang
Yu, Qian
Upur, Halmurat
author_sort Tian, Shuge
collection PubMed
description A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of oleanolic acid and ursolic acid contents in the Ziziphora clinopodioides Lam. with short run time. Chromatographic separation is achieved by using HPLC system consisting of a Shimadzu LC-6AD and Kromasil C(18) column (150 × 4.6 mm, 10 μm, with pre-column), the mobile phase consists of methanol and 0.03 M phosphate buffer (pH = 3, 90:10). Detection wavelength is 214 nm. The speed of flow is 0.5 ml/min. The specimen handing quantity is 10 μl. The oleanolic acid's linearity range is 0.4 ~ 1.2 mg/ml (r = 0.9996). The ursolic acid's linearity range is 0.6 ~ 1.8 mg/ml (r = 0.9996), and the linear relationship is accurate. The average recovery (n = 6) of oleanolic acid is 99.5% (RSD = 1.19%) and ursolic acid is 102.3% (RSD = 1.25%). The content of oleanolic acid and ursolic acid in Ziziphora clinopodioides are 0.76 mg/g and 1.176 mg/g, respectively. The developed HPLC method can therefore be applied to both in vitro studies of oleanolic acid and ursolic acid formulations as well as drug estimation in biological samples.
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spelling pubmed-29000582010-07-28 Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method Tian, Shuge Shi, Yang Yu, Qian Upur, Halmurat Pharmacogn Mag Original Article A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of oleanolic acid and ursolic acid contents in the Ziziphora clinopodioides Lam. with short run time. Chromatographic separation is achieved by using HPLC system consisting of a Shimadzu LC-6AD and Kromasil C(18) column (150 × 4.6 mm, 10 μm, with pre-column), the mobile phase consists of methanol and 0.03 M phosphate buffer (pH = 3, 90:10). Detection wavelength is 214 nm. The speed of flow is 0.5 ml/min. The specimen handing quantity is 10 μl. The oleanolic acid's linearity range is 0.4 ~ 1.2 mg/ml (r = 0.9996). The ursolic acid's linearity range is 0.6 ~ 1.8 mg/ml (r = 0.9996), and the linear relationship is accurate. The average recovery (n = 6) of oleanolic acid is 99.5% (RSD = 1.19%) and ursolic acid is 102.3% (RSD = 1.25%). The content of oleanolic acid and ursolic acid in Ziziphora clinopodioides are 0.76 mg/g and 1.176 mg/g, respectively. The developed HPLC method can therefore be applied to both in vitro studies of oleanolic acid and ursolic acid formulations as well as drug estimation in biological samples. Medknow Publications 2010-04 2010-05-05 /pmc/articles/PMC2900058/ /pubmed/20668577 http://dx.doi.org/10.4103/0973-1296.62898 Text en © Pharmacognosy Magazine http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tian, Shuge
Shi, Yang
Yu, Qian
Upur, Halmurat
Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method
title Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method
title_full Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method
title_fullStr Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method
title_full_unstemmed Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method
title_short Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method
title_sort determination of oleanolic acid and ursolic acid contents in ziziphora clinopodioides lam. by hplc method
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900058/
https://www.ncbi.nlm.nih.gov/pubmed/20668577
http://dx.doi.org/10.4103/0973-1296.62898
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