Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography

A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm × 50 mm, 1.8 μm) col...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Xiaoying, Chen, Xiao-Ming, Ge, Liang, Gong, Hai-Yan, Tian, Shuge, An, Dongqing
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900069/
https://www.ncbi.nlm.nih.gov/pubmed/20668573
http://dx.doi.org/10.4103/0973-1296.62893
Descripción
Sumario:A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm × 50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.

Ejemplares similares