Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography

A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm × 50 mm, 1.8 μm) col...

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Detalles Bibliográficos
Autores principales: Zhou, Xiaoying, Chen, Xiao-Ming, Ge, Liang, Gong, Hai-Yan, Tian, Shuge, An, Dongqing
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900069/
https://www.ncbi.nlm.nih.gov/pubmed/20668573
http://dx.doi.org/10.4103/0973-1296.62893
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author Zhou, Xiaoying
Chen, Xiao-Ming
Ge, Liang
Gong, Hai-Yan
Tian, Shuge
An, Dongqing
author_facet Zhou, Xiaoying
Chen, Xiao-Ming
Ge, Liang
Gong, Hai-Yan
Tian, Shuge
An, Dongqing
author_sort Zhou, Xiaoying
collection PubMed
description A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm × 50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.
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spelling pubmed-29000692010-07-28 Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography Zhou, Xiaoying Chen, Xiao-Ming Ge, Liang Gong, Hai-Yan Tian, Shuge An, Dongqing Pharmacogn Mag Original Article A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm × 50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples. Medknow Publications 2010-04 2010-05-05 /pmc/articles/PMC2900069/ /pubmed/20668573 http://dx.doi.org/10.4103/0973-1296.62893 Text en © Pharmacognosy Magazine http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhou, Xiaoying
Chen, Xiao-Ming
Ge, Liang
Gong, Hai-Yan
Tian, Shuge
An, Dongqing
Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
title Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
title_full Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
title_fullStr Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
title_full_unstemmed Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
title_short Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
title_sort rapid determination of paeoniflorin from paeonia sinjiang k. y. pan. by rapid resolution liquid chromatography
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900069/
https://www.ncbi.nlm.nih.gov/pubmed/20668573
http://dx.doi.org/10.4103/0973-1296.62893
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