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Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer Netherlands
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900597/ https://www.ncbi.nlm.nih.gov/pubmed/19816792 http://dx.doi.org/10.1007/s11033-009-9849-z |
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author | Moyano, Eva M. González, Luis Miguel Cuevas, Laureano Perez-Pastrana, Esperanza Santa-Maria, Ysmael Benito, Agustín |
author_facet | Moyano, Eva M. González, Luis Miguel Cuevas, Laureano Perez-Pastrana, Esperanza Santa-Maria, Ysmael Benito, Agustín |
author_sort | Moyano, Eva M. |
collection | PubMed |
description | To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay’s sensitivity and specificity were 78.2 and 94% respectively. |
format | Text |
id | pubmed-2900597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-29005972010-07-30 Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum Moyano, Eva M. González, Luis Miguel Cuevas, Laureano Perez-Pastrana, Esperanza Santa-Maria, Ysmael Benito, Agustín Mol Biol Rep Article To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay’s sensitivity and specificity were 78.2 and 94% respectively. Springer Netherlands 2009-10-09 2010 /pmc/articles/PMC2900597/ /pubmed/19816792 http://dx.doi.org/10.1007/s11033-009-9849-z Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Moyano, Eva M. González, Luis Miguel Cuevas, Laureano Perez-Pastrana, Esperanza Santa-Maria, Ysmael Benito, Agustín Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum |
title | Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum |
title_full | Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum |
title_fullStr | Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum |
title_full_unstemmed | Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum |
title_short | Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum |
title_sort | molecular cloning and characterisation of the resa gene, a marker of genetic diversity of plasmodium falciparum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900597/ https://www.ncbi.nlm.nih.gov/pubmed/19816792 http://dx.doi.org/10.1007/s11033-009-9849-z |
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