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Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum

To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit...

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Autores principales: Moyano, Eva M., González, Luis Miguel, Cuevas, Laureano, Perez-Pastrana, Esperanza, Santa-Maria, Ysmael, Benito, Agustín
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900597/
https://www.ncbi.nlm.nih.gov/pubmed/19816792
http://dx.doi.org/10.1007/s11033-009-9849-z
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author Moyano, Eva M.
González, Luis Miguel
Cuevas, Laureano
Perez-Pastrana, Esperanza
Santa-Maria, Ysmael
Benito, Agustín
author_facet Moyano, Eva M.
González, Luis Miguel
Cuevas, Laureano
Perez-Pastrana, Esperanza
Santa-Maria, Ysmael
Benito, Agustín
author_sort Moyano, Eva M.
collection PubMed
description To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay’s sensitivity and specificity were 78.2 and 94% respectively.
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spelling pubmed-29005972010-07-30 Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum Moyano, Eva M. González, Luis Miguel Cuevas, Laureano Perez-Pastrana, Esperanza Santa-Maria, Ysmael Benito, Agustín Mol Biol Rep Article To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay’s sensitivity and specificity were 78.2 and 94% respectively. Springer Netherlands 2009-10-09 2010 /pmc/articles/PMC2900597/ /pubmed/19816792 http://dx.doi.org/10.1007/s11033-009-9849-z Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Moyano, Eva M.
González, Luis Miguel
Cuevas, Laureano
Perez-Pastrana, Esperanza
Santa-Maria, Ysmael
Benito, Agustín
Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
title Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
title_full Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
title_fullStr Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
title_full_unstemmed Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
title_short Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
title_sort molecular cloning and characterisation of the resa gene, a marker of genetic diversity of plasmodium falciparum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900597/
https://www.ncbi.nlm.nih.gov/pubmed/19816792
http://dx.doi.org/10.1007/s11033-009-9849-z
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