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The development of meibomian glands in mice
PURPOSE: The purpose of this study was to characterize the natural history of meibomian gland morphogenesis in the mouse. METHODS: Embryonic (E) and post natal (P) C57Bl/6 mouse pups were obtained at E18.5, P0, P1, P3, P5, P8, P15, and P60. Eyelids were fixed and processed for en bloc staining with...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Vision
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901193/ https://www.ncbi.nlm.nih.gov/pubmed/20664693 |
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author | Nien, Chyong Jy Massei, Salina Lin, Gloria Liu, Hongshan Paugh, Jerry R. Liu, Chia-Yang Kao, Winston Whei-Yang Brown, Donald J. Jester, James V. |
author_facet | Nien, Chyong Jy Massei, Salina Lin, Gloria Liu, Hongshan Paugh, Jerry R. Liu, Chia-Yang Kao, Winston Whei-Yang Brown, Donald J. Jester, James V. |
author_sort | Nien, Chyong Jy |
collection | PubMed |
description | PURPOSE: The purpose of this study was to characterize the natural history of meibomian gland morphogenesis in the mouse. METHODS: Embryonic (E) and post natal (P) C57Bl/6 mouse pups were obtained at E18.5, P0, P1, P3, P5, P8, P15, and P60. Eyelids were fixed and processed for en bloc staining with Phalloidin/DAPI to identify gland morphogenesis, or frozen for immunohistochemistry staining with Oil red O (ORO) to identify lipid and antibodies specific against peroxisome proliferator-activated receptor gamma (PPARγ) to identify meibocyte differentiation. Samples were then evaluated using a Zeiss 510 Meta laser scanning confocal microscope or Nikon epi-fluorescent microscope. Tissues from adult mice (2 month-old) were also collected for western blotting. RESULTS: Meibomian gland morphogenesis was first detected at E18.5 with the formation of an epithelial placode within the fused eyelid margin. Invagination of the epithelium into the eyelid was detected at P0. From P1 to P3 there was continued extension of the epithelium into the eyelid. ORO and PPARγ staining was first detected at P3, localized to the central core of the epithelial cord thus forming the presumptive ductal lumen. Ductal branching was first detected at P5 associated with acinar differentiation identified by ORO and PPARγ staining. Adult meibomian glands were observed by P15. Western blotting of meibomian gland proteins identified a 50 kDa and a 72 kDa band that stained with antibodies specific to PPARγ. CONCLUSIONS: We have demonstrated that meibomian gland development bears distinct similarities to hair development with the formation of an epithelial placode and expression of PPARγ co-incident with lipid synthesis and meibocyte differentiation. |
format | Text |
id | pubmed-2901193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-29011932010-07-21 The development of meibomian glands in mice Nien, Chyong Jy Massei, Salina Lin, Gloria Liu, Hongshan Paugh, Jerry R. Liu, Chia-Yang Kao, Winston Whei-Yang Brown, Donald J. Jester, James V. Mol Vis Research Article PURPOSE: The purpose of this study was to characterize the natural history of meibomian gland morphogenesis in the mouse. METHODS: Embryonic (E) and post natal (P) C57Bl/6 mouse pups were obtained at E18.5, P0, P1, P3, P5, P8, P15, and P60. Eyelids were fixed and processed for en bloc staining with Phalloidin/DAPI to identify gland morphogenesis, or frozen for immunohistochemistry staining with Oil red O (ORO) to identify lipid and antibodies specific against peroxisome proliferator-activated receptor gamma (PPARγ) to identify meibocyte differentiation. Samples were then evaluated using a Zeiss 510 Meta laser scanning confocal microscope or Nikon epi-fluorescent microscope. Tissues from adult mice (2 month-old) were also collected for western blotting. RESULTS: Meibomian gland morphogenesis was first detected at E18.5 with the formation of an epithelial placode within the fused eyelid margin. Invagination of the epithelium into the eyelid was detected at P0. From P1 to P3 there was continued extension of the epithelium into the eyelid. ORO and PPARγ staining was first detected at P3, localized to the central core of the epithelial cord thus forming the presumptive ductal lumen. Ductal branching was first detected at P5 associated with acinar differentiation identified by ORO and PPARγ staining. Adult meibomian glands were observed by P15. Western blotting of meibomian gland proteins identified a 50 kDa and a 72 kDa band that stained with antibodies specific to PPARγ. CONCLUSIONS: We have demonstrated that meibomian gland development bears distinct similarities to hair development with the formation of an epithelial placode and expression of PPARγ co-incident with lipid synthesis and meibocyte differentiation. Molecular Vision 2010-06-18 /pmc/articles/PMC2901193/ /pubmed/20664693 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nien, Chyong Jy Massei, Salina Lin, Gloria Liu, Hongshan Paugh, Jerry R. Liu, Chia-Yang Kao, Winston Whei-Yang Brown, Donald J. Jester, James V. The development of meibomian glands in mice |
title | The development of meibomian glands in mice |
title_full | The development of meibomian glands in mice |
title_fullStr | The development of meibomian glands in mice |
title_full_unstemmed | The development of meibomian glands in mice |
title_short | The development of meibomian glands in mice |
title_sort | development of meibomian glands in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901193/ https://www.ncbi.nlm.nih.gov/pubmed/20664693 |
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