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Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

BACKGROUND: Technology used for the purification of recombinant proteins is a key issue for the biochemical and structural analyses of proteins. In general, affinity tags, such as glutathione-S-transferase or six-histidines, are used to purify recombinant proteins. Since such affinity tags often int...

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Detalles Bibliográficos
Autores principales:	Matsunaga, Satoko, Matsuoka, Kazuhiro, Shimizu, Kouhei, Endo, Yaeta, Sawasaki, Tatsuya
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901362/ https://www.ncbi.nlm.nih.gov/pubmed/20525307 http://dx.doi.org/10.1186/1472-6750-10-42

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