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Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model

Curcumin has gained immense importance for its vast therapeutic and prophylactic applications. Contrary to this, our study reveals that it regulates the defense pathways of Salmonella enterica serovar Typhimurium (S. Typhimurium) to enhance its pathogenicity. In a murine model of typhoid fever, we o...

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Autores principales: Marathe, Sandhya A., Ray, Seemun, Chakravortty, Dipshikha
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901387/
https://www.ncbi.nlm.nih.gov/pubmed/20634977
http://dx.doi.org/10.1371/journal.pone.0011511
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author Marathe, Sandhya A.
Ray, Seemun
Chakravortty, Dipshikha
author_facet Marathe, Sandhya A.
Ray, Seemun
Chakravortty, Dipshikha
author_sort Marathe, Sandhya A.
collection PubMed
description Curcumin has gained immense importance for its vast therapeutic and prophylactic applications. Contrary to this, our study reveals that it regulates the defense pathways of Salmonella enterica serovar Typhimurium (S. Typhimurium) to enhance its pathogenicity. In a murine model of typhoid fever, we observed higher bacterial load in Peyer's patches, mesenteric lymph node, spleen and liver, when infected with curcumin-treated Salmonella. Curcumin increased the resistance of S. Typhimurium against antimicrobial agents like antimicrobial peptides, reactive oxygen and nitrogen species. This increased tolerance might be attributed to the up-regulation of genes involved in resistance against antimicrobial peptides - pmrD and pmrHFIJKLM and genes with antioxidant function - mntH, sodA and sitA. We implicate that iron chelation property of curcumin have a role in regulating mntH and sitA. Interestingly, we see that the curcumin-mediated modulation of pmr genes is through the PhoPQ regulatory system. Curcumin downregulates SPI1 genes, required for entry into epithelial cells and upregulates SPI2 genes required to intracellular survival. Since it is known that the SPI1 and SPI2 system can be regulated by the PhoPQ system, this common regulator could explain curcumin's mode of action. This data urges us to rethink the indiscriminate use of curcumin especially during Salmonella outbreaks.
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spelling pubmed-29013872010-07-15 Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model Marathe, Sandhya A. Ray, Seemun Chakravortty, Dipshikha PLoS One Research Article Curcumin has gained immense importance for its vast therapeutic and prophylactic applications. Contrary to this, our study reveals that it regulates the defense pathways of Salmonella enterica serovar Typhimurium (S. Typhimurium) to enhance its pathogenicity. In a murine model of typhoid fever, we observed higher bacterial load in Peyer's patches, mesenteric lymph node, spleen and liver, when infected with curcumin-treated Salmonella. Curcumin increased the resistance of S. Typhimurium against antimicrobial agents like antimicrobial peptides, reactive oxygen and nitrogen species. This increased tolerance might be attributed to the up-regulation of genes involved in resistance against antimicrobial peptides - pmrD and pmrHFIJKLM and genes with antioxidant function - mntH, sodA and sitA. We implicate that iron chelation property of curcumin have a role in regulating mntH and sitA. Interestingly, we see that the curcumin-mediated modulation of pmr genes is through the PhoPQ regulatory system. Curcumin downregulates SPI1 genes, required for entry into epithelial cells and upregulates SPI2 genes required to intracellular survival. Since it is known that the SPI1 and SPI2 system can be regulated by the PhoPQ system, this common regulator could explain curcumin's mode of action. This data urges us to rethink the indiscriminate use of curcumin especially during Salmonella outbreaks. Public Library of Science 2010-07-09 /pmc/articles/PMC2901387/ /pubmed/20634977 http://dx.doi.org/10.1371/journal.pone.0011511 Text en Marathe et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Marathe, Sandhya A.
Ray, Seemun
Chakravortty, Dipshikha
Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model
title Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model
title_full Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model
title_fullStr Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model
title_full_unstemmed Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model
title_short Curcumin Increases the Pathogenicity of Salmonella enterica Serovar Typhimurium in Murine Model
title_sort curcumin increases the pathogenicity of salmonella enterica serovar typhimurium in murine model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901387/
https://www.ncbi.nlm.nih.gov/pubmed/20634977
http://dx.doi.org/10.1371/journal.pone.0011511
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