Cargando…
Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites
The rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overc...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902127/ https://www.ncbi.nlm.nih.gov/pubmed/20634989 http://dx.doi.org/10.1155/2010/820476 |
_version_ | 1782183742033362944 |
---|---|
author | Spyranti, Zinovia Galanis, Athanassios S. Pairas, George Spyroulias, Georgios A. Manessi-Zoupa, Evy Cordopatis, Paul |
author_facet | Spyranti, Zinovia Galanis, Athanassios S. Pairas, George Spyroulias, Georgios A. Manessi-Zoupa, Evy Cordopatis, Paul |
author_sort | Spyranti, Zinovia |
collection | PubMed |
description | The rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overcome for the determination of the high resolution structure was the crystallization of the highly hydrophobic transmembrane domain. Following our previous work, synthetic peptides and Zinc(II) metal ions are used to build structural maquettes of the two Zn-catalytic active sites of the ACE somatic isoform. Structural investigations of the synthetic peptides, representing the two different somatic isoform active sites, through circular dichroism and NMR experiments are reported. |
format | Text |
id | pubmed-2902127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29021272010-07-15 Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites Spyranti, Zinovia Galanis, Athanassios S. Pairas, George Spyroulias, Georgios A. Manessi-Zoupa, Evy Cordopatis, Paul Bioinorg Chem Appl Research Article The rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overcome for the determination of the high resolution structure was the crystallization of the highly hydrophobic transmembrane domain. Following our previous work, synthetic peptides and Zinc(II) metal ions are used to build structural maquettes of the two Zn-catalytic active sites of the ACE somatic isoform. Structural investigations of the synthetic peptides, representing the two different somatic isoform active sites, through circular dichroism and NMR experiments are reported. Hindawi Publishing Corporation 2010 2010-06-09 /pmc/articles/PMC2902127/ /pubmed/20634989 http://dx.doi.org/10.1155/2010/820476 Text en Copyright © 2010 Zinovia Spyranti et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Spyranti, Zinovia Galanis, Athanassios S. Pairas, George Spyroulias, Georgios A. Manessi-Zoupa, Evy Cordopatis, Paul Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
title | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
title_full | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
title_fullStr | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
title_full_unstemmed | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
title_short | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
title_sort | synthetic peptides as structural maquettes of angiotensin-i converting enzyme catalytic sites |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902127/ https://www.ncbi.nlm.nih.gov/pubmed/20634989 http://dx.doi.org/10.1155/2010/820476 |
work_keys_str_mv | AT spyrantizinovia syntheticpeptidesasstructuralmaquettesofangiotensiniconvertingenzymecatalyticsites AT galanisathanassioss syntheticpeptidesasstructuralmaquettesofangiotensiniconvertingenzymecatalyticsites AT pairasgeorge syntheticpeptidesasstructuralmaquettesofangiotensiniconvertingenzymecatalyticsites AT spyrouliasgeorgiosa syntheticpeptidesasstructuralmaquettesofangiotensiniconvertingenzymecatalyticsites AT manessizoupaevy syntheticpeptidesasstructuralmaquettesofangiotensiniconvertingenzymecatalyticsites AT cordopatispaul syntheticpeptidesasstructuralmaquettesofangiotensiniconvertingenzymecatalyticsites |