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Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft

BACKGROUND: In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model. METHODS: The potential of alpha-TCP on bone repair was compared to autogenous bone grafting,...

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Autores principales: Puricelli, Edela, Corsetti, Adriana, Ponzoni, Deise, Martins, Gustavo L, Leite, Mauro G, Santos, Luis A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902431/
https://www.ncbi.nlm.nih.gov/pubmed/20579394
http://dx.doi.org/10.1186/1746-160X-6-10
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author Puricelli, Edela
Corsetti, Adriana
Ponzoni, Deise
Martins, Gustavo L
Leite, Mauro G
Santos, Luis A
author_facet Puricelli, Edela
Corsetti, Adriana
Ponzoni, Deise
Martins, Gustavo L
Leite, Mauro G
Santos, Luis A
author_sort Puricelli, Edela
collection PubMed
description BACKGROUND: In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model. METHODS: The potential of alpha-TCP on bone repair was compared to autogenous bone grafting, and unfilled cavities were used as negative control. Surgical cavities were prepared and designated as test (T), implanted with alpha-TCP blocks; negative control (C - ), unfilled; and positive control (C + ), implanted with autogenous bone graft. Results were analyzed on postoperative days three, seven, 14, 21 and 60. RESULTS: The histological analyses showed the following results. Postoperative day three: presence of inflammatory infiltrate, erythrocytes and proliferating fibroblasts in T, C - and C + samples. Day seven: extensive bone neoformation in groups T and C + , and beginning of alpha-TCP resorption by phagocytic cells. Days 14 and 21: osteoblastic activity in the three types of cavities. Day 60: In all samples, neoformed bone similar to surrounding bone. Moderate interruption on the ostectomized cortical bone. CONCLUSIONS: Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone. Comparison of C - with T and C + samples showed that repair is faster in implanted cavities; on day 60, control groups presented almost complete bone repair. Alpha-TCP cement presents biocompatibility and osteotransductivity, besides stability, but 60 days after surgery the cavities were not closed.
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spelling pubmed-29024312010-07-13 Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft Puricelli, Edela Corsetti, Adriana Ponzoni, Deise Martins, Gustavo L Leite, Mauro G Santos, Luis A Head Face Med Research BACKGROUND: In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model. METHODS: The potential of alpha-TCP on bone repair was compared to autogenous bone grafting, and unfilled cavities were used as negative control. Surgical cavities were prepared and designated as test (T), implanted with alpha-TCP blocks; negative control (C - ), unfilled; and positive control (C + ), implanted with autogenous bone graft. Results were analyzed on postoperative days three, seven, 14, 21 and 60. RESULTS: The histological analyses showed the following results. Postoperative day three: presence of inflammatory infiltrate, erythrocytes and proliferating fibroblasts in T, C - and C + samples. Day seven: extensive bone neoformation in groups T and C + , and beginning of alpha-TCP resorption by phagocytic cells. Days 14 and 21: osteoblastic activity in the three types of cavities. Day 60: In all samples, neoformed bone similar to surrounding bone. Moderate interruption on the ostectomized cortical bone. CONCLUSIONS: Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone. Comparison of C - with T and C + samples showed that repair is faster in implanted cavities; on day 60, control groups presented almost complete bone repair. Alpha-TCP cement presents biocompatibility and osteotransductivity, besides stability, but 60 days after surgery the cavities were not closed. BioMed Central 2010-06-28 /pmc/articles/PMC2902431/ /pubmed/20579394 http://dx.doi.org/10.1186/1746-160X-6-10 Text en Copyright ©2010 Puricelli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Puricelli, Edela
Corsetti, Adriana
Ponzoni, Deise
Martins, Gustavo L
Leite, Mauro G
Santos, Luis A
Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
title Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
title_full Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
title_fullStr Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
title_full_unstemmed Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
title_short Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
title_sort characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902431/
https://www.ncbi.nlm.nih.gov/pubmed/20579394
http://dx.doi.org/10.1186/1746-160X-6-10
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