Cargando…
Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design
BACKGROUND: Dendritic Cells (DC) are potent antigen presenting cells with the ability to prime naïve T cells and convert them to cytotoxic T-lymphocytes (CTL). We evaluated the capability of autologous DCs transfected with total tumor and normal RNA to induce cytotoxic CTL as the preliminary step to...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902443/ https://www.ncbi.nlm.nih.gov/pubmed/20525404 http://dx.doi.org/10.1186/1471-2407-10-261 |
_version_ | 1782183761176166400 |
---|---|
author | Gholamin, Mehran Moaven, Omeed Farshchian, Moein Mahmoudi, Mahmoud Sankian, Mojtaba Memar, Bahram Forghani, Mohammad Naser Malekzadeh, Reza Rajabi-Mashhadi, Mohammad Taghi Abbaszadegan, Mohammad Reza |
author_facet | Gholamin, Mehran Moaven, Omeed Farshchian, Moein Mahmoudi, Mahmoud Sankian, Mojtaba Memar, Bahram Forghani, Mohammad Naser Malekzadeh, Reza Rajabi-Mashhadi, Mohammad Taghi Abbaszadegan, Mohammad Reza |
author_sort | Gholamin, Mehran |
collection | PubMed |
description | BACKGROUND: Dendritic Cells (DC) are potent antigen presenting cells with the ability to prime naïve T cells and convert them to cytotoxic T-lymphocytes (CTL). We evaluated the capability of autologous DCs transfected with total tumor and normal RNA to induce cytotoxic CTL as the preliminary step to design a DC-based vaccine in the esophageal squamous cell carcinoma (ESCC). METHODS: Monocytes-derived DCs were electroporated with either total tumor RNA or normal RNA. T cells were then primed with tumor RNA transfected DCs and lytic effects of the generated CTL were measured with Cytotoxicity assay and IFN-γ Release Elispot assay. RESULTS: Cytotoxicity was induced against DCs loaded with tumoral RNA (%24.8 ± 5.2 SEM) while in normal RNA-loaded DCs, it was minimal (%6.1 ± 2.4 SEM) and significantly lower (p < 0.05). INF-γ secretion was more than 2-folds higher in tumoral RNA-loaded DCs when compared with normal RNA-loaded DCs (p < 0.05). CONCLUSION: Electroporating DCs with tumor RNA generated tumor antigen presenting cells which in turn enhanced cytotoxic effects of the T cells against ESCC. This may be a useful autologous ex vivo screening tool for confirming the lytic effects of primed T cells on tumors and evaluate probable further adverse effects on noncancerous tissues. These data provide crucial preliminary information to establish a total tumor RNA-pulsed DC vaccine therapy of ESCC. |
format | Text |
id | pubmed-2902443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29024432010-07-13 Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design Gholamin, Mehran Moaven, Omeed Farshchian, Moein Mahmoudi, Mahmoud Sankian, Mojtaba Memar, Bahram Forghani, Mohammad Naser Malekzadeh, Reza Rajabi-Mashhadi, Mohammad Taghi Abbaszadegan, Mohammad Reza BMC Cancer Research Article BACKGROUND: Dendritic Cells (DC) are potent antigen presenting cells with the ability to prime naïve T cells and convert them to cytotoxic T-lymphocytes (CTL). We evaluated the capability of autologous DCs transfected with total tumor and normal RNA to induce cytotoxic CTL as the preliminary step to design a DC-based vaccine in the esophageal squamous cell carcinoma (ESCC). METHODS: Monocytes-derived DCs were electroporated with either total tumor RNA or normal RNA. T cells were then primed with tumor RNA transfected DCs and lytic effects of the generated CTL were measured with Cytotoxicity assay and IFN-γ Release Elispot assay. RESULTS: Cytotoxicity was induced against DCs loaded with tumoral RNA (%24.8 ± 5.2 SEM) while in normal RNA-loaded DCs, it was minimal (%6.1 ± 2.4 SEM) and significantly lower (p < 0.05). INF-γ secretion was more than 2-folds higher in tumoral RNA-loaded DCs when compared with normal RNA-loaded DCs (p < 0.05). CONCLUSION: Electroporating DCs with tumor RNA generated tumor antigen presenting cells which in turn enhanced cytotoxic effects of the T cells against ESCC. This may be a useful autologous ex vivo screening tool for confirming the lytic effects of primed T cells on tumors and evaluate probable further adverse effects on noncancerous tissues. These data provide crucial preliminary information to establish a total tumor RNA-pulsed DC vaccine therapy of ESCC. BioMed Central 2010-06-07 /pmc/articles/PMC2902443/ /pubmed/20525404 http://dx.doi.org/10.1186/1471-2407-10-261 Text en Copyright ©2010 Gholamin et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gholamin, Mehran Moaven, Omeed Farshchian, Moein Mahmoudi, Mahmoud Sankian, Mojtaba Memar, Bahram Forghani, Mohammad Naser Malekzadeh, Reza Rajabi-Mashhadi, Mohammad Taghi Abbaszadegan, Mohammad Reza Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design |
title | Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design |
title_full | Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design |
title_fullStr | Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design |
title_full_unstemmed | Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design |
title_short | Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: preliminary step for DC vaccine design |
title_sort | induction of cytotoxic t lymphocytes primed with tumor rna-loaded dendritic cells in esophageal squamous cell carcinoma: preliminary step for dc vaccine design |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902443/ https://www.ncbi.nlm.nih.gov/pubmed/20525404 http://dx.doi.org/10.1186/1471-2407-10-261 |
work_keys_str_mv | AT gholaminmehran inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT moavenomeed inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT farshchianmoein inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT mahmoudimahmoud inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT sankianmojtaba inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT memarbahram inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT forghanimohammadnaser inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT malekzadehreza inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT rajabimashhadimohammadtaghi inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign AT abbaszadeganmohammadreza inductionofcytotoxictlymphocytesprimedwithtumorrnaloadeddendriticcellsinesophagealsquamouscellcarcinomapreliminarystepfordcvaccinedesign |