Could protein tertiary structure influence mammary transgene expression more than tissue specific codon usage?

Animal mammary glands have been successfully employed to produce therapeutic recombinant human proteins. However, considerable variation in animal mammary transgene expression efficiency has been reported. We now consider whether aspects of codon usage and/or protein tertiary structure underlie this...

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Detalles Bibliográficos
Autores principales: He, Zuyong, Zhao, Yiqiang, Mei, Gui, Li, Ning, Chen, Yaosheng
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902731/
https://www.ncbi.nlm.nih.gov/pubmed/20563642
http://dx.doi.org/10.1007/s11248-010-9411-8
Descripción
Sumario:Animal mammary glands have been successfully employed to produce therapeutic recombinant human proteins. However, considerable variation in animal mammary transgene expression efficiency has been reported. We now consider whether aspects of codon usage and/or protein tertiary structure underlie this variation in mammary transgene expression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11248-010-9411-8) contains supplementary material, which is available to authorized users.

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