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Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium

BACKGROUND: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by...

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Autores principales: Posadas, Sinforiano J, Caz, Victor, Caballero, Isabel, Cendejas, Emilio, Quilez, Immaculada, Largo, Carlota, Elvira, Marcos, De Miguel, Enrique
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2903505/
https://www.ncbi.nlm.nih.gov/pubmed/20529359
http://dx.doi.org/10.1186/1471-230X-10-58
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author Posadas, Sinforiano J
Caz, Victor
Caballero, Isabel
Cendejas, Emilio
Quilez, Immaculada
Largo, Carlota
Elvira, Marcos
De Miguel, Enrique
author_facet Posadas, Sinforiano J
Caz, Victor
Caballero, Isabel
Cendejas, Emilio
Quilez, Immaculada
Largo, Carlota
Elvira, Marcos
De Miguel, Enrique
author_sort Posadas, Sinforiano J
collection PubMed
description BACKGROUND: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria. This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium. METHODS: Adult Wistar male rats were divided into three groups: control, and mannoprotein E(1 )at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E(1). Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry. RESULTS: Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent. CONCLUSIONS: Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.
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spelling pubmed-29035052010-07-14 Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium Posadas, Sinforiano J Caz, Victor Caballero, Isabel Cendejas, Emilio Quilez, Immaculada Largo, Carlota Elvira, Marcos De Miguel, Enrique BMC Gastroenterol Research Article BACKGROUND: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria. This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium. METHODS: Adult Wistar male rats were divided into three groups: control, and mannoprotein E(1 )at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E(1). Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry. RESULTS: Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent. CONCLUSIONS: Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia. BioMed Central 2010-06-08 /pmc/articles/PMC2903505/ /pubmed/20529359 http://dx.doi.org/10.1186/1471-230X-10-58 Text en Copyright ©2010 Posadas et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Posadas, Sinforiano J
Caz, Victor
Caballero, Isabel
Cendejas, Emilio
Quilez, Immaculada
Largo, Carlota
Elvira, Marcos
De Miguel, Enrique
Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium
title Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium
title_full Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium
title_fullStr Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium
title_full_unstemmed Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium
title_short Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium
title_sort effects of mannoprotein e1 in liquid diet on inflammatory response and tlr5 expression in the gut of rats infected by salmonella typhimurium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2903505/
https://www.ncbi.nlm.nih.gov/pubmed/20529359
http://dx.doi.org/10.1186/1471-230X-10-58
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