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Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study

INTRODUCTION: The percentage of neutrophils in sputum are increased in COPD patients, and may therefore be a biomarker of airway inflammation. We studied the relationships between sputum neutrophils and FEV(1), health status, exacerbation rates, systemic inflammation and emphysema, and long term var...

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Autores principales: Singh, Dave, Edwards, Lisa, Tal-Singer, Ruth, Rennard, Stephen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904285/
https://www.ncbi.nlm.nih.gov/pubmed/20550701
http://dx.doi.org/10.1186/1465-9921-11-77
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author Singh, Dave
Edwards, Lisa
Tal-Singer, Ruth
Rennard, Stephen
author_facet Singh, Dave
Edwards, Lisa
Tal-Singer, Ruth
Rennard, Stephen
author_sort Singh, Dave
collection PubMed
description INTRODUCTION: The percentage of neutrophils in sputum are increased in COPD patients, and may therefore be a biomarker of airway inflammation. We studied the relationships between sputum neutrophils and FEV(1), health status, exacerbation rates, systemic inflammation and emphysema, and long term variability at 1 year. METHODS: Sputum samples were obtained from 488 COPD patients within the ECLIPSE cohort. 359 samples were obtained at baseline, and 297 after 1 year. 168 subjects provided samples at both visits. Serum interleukin-6 (IL-6), IL-8, surfactant protein D and C-reactive protein levels were measured by immunoassays. Low-dose CT scans evaluated emphysema. RESULTS: Sputum neutrophil % increased with GOLD stage. There was a weak association between % sputum neutrophils and FEV(1 )% predicted (univariate r(2 )= 0.025 and 0.094 at baseline and year 1 respectively, p < 0.05 after multivariate regression). Similar weak but significant associations were observed between neutrophil % and health status measured using the St Georges Respiratory Questionairre. There were no associations between neutrophils and exacerbation rates or emphysema. Associations between sputum neutrophils and systemic biomarkers were non-significant or similarly weak. The mean change over 1 year in neutrophil % was an increase of 3.5%. CONCLUSIONS: Sputum neutrophil measurements in COPD are associated weakly with FEV(1 )% predicted and health status. Sputum neutrophil measurements were dissociated from exacerbation rates, emphysema and systemic inflammation.
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spelling pubmed-29042852010-07-15 Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study Singh, Dave Edwards, Lisa Tal-Singer, Ruth Rennard, Stephen Respir Res Research INTRODUCTION: The percentage of neutrophils in sputum are increased in COPD patients, and may therefore be a biomarker of airway inflammation. We studied the relationships between sputum neutrophils and FEV(1), health status, exacerbation rates, systemic inflammation and emphysema, and long term variability at 1 year. METHODS: Sputum samples were obtained from 488 COPD patients within the ECLIPSE cohort. 359 samples were obtained at baseline, and 297 after 1 year. 168 subjects provided samples at both visits. Serum interleukin-6 (IL-6), IL-8, surfactant protein D and C-reactive protein levels were measured by immunoassays. Low-dose CT scans evaluated emphysema. RESULTS: Sputum neutrophil % increased with GOLD stage. There was a weak association between % sputum neutrophils and FEV(1 )% predicted (univariate r(2 )= 0.025 and 0.094 at baseline and year 1 respectively, p < 0.05 after multivariate regression). Similar weak but significant associations were observed between neutrophil % and health status measured using the St Georges Respiratory Questionairre. There were no associations between neutrophils and exacerbation rates or emphysema. Associations between sputum neutrophils and systemic biomarkers were non-significant or similarly weak. The mean change over 1 year in neutrophil % was an increase of 3.5%. CONCLUSIONS: Sputum neutrophil measurements in COPD are associated weakly with FEV(1 )% predicted and health status. Sputum neutrophil measurements were dissociated from exacerbation rates, emphysema and systemic inflammation. BioMed Central 2010 2010-06-15 /pmc/articles/PMC2904285/ /pubmed/20550701 http://dx.doi.org/10.1186/1465-9921-11-77 Text en Copyright ©2010 Singh et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Singh, Dave
Edwards, Lisa
Tal-Singer, Ruth
Rennard, Stephen
Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study
title Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study
title_full Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study
title_fullStr Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study
title_full_unstemmed Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study
title_short Sputum neutrophils as a biomarker in COPD: findings from the ECLIPSE study
title_sort sputum neutrophils as a biomarker in copd: findings from the eclipse study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904285/
https://www.ncbi.nlm.nih.gov/pubmed/20550701
http://dx.doi.org/10.1186/1465-9921-11-77
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