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A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a

BACKGROUND: Shigella flexneri is a gram-negative, facultative pathogen that causes the majority of communicable bacterial dysenteries in developing countries. The virulence factors of S. flexneri have been shown to be produced at 37 degrees C but not at 30 degrees C. To discover potential, novel vir...

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Autores principales: Zhao, Ge, Zhu, Li, Feng, Erling, Cao, Xiaoyu, Shang, Na, Liu, Xiankai, Liao, Xiang, Ying, Tianyi, Wang, Jie, Chen, Huipeng, Wang, Hengliang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904734/
https://www.ncbi.nlm.nih.gov/pubmed/20540790
http://dx.doi.org/10.1186/1477-5956-8-30
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author Zhao, Ge
Zhu, Li
Feng, Erling
Cao, Xiaoyu
Shang, Na
Liu, Xiankai
Liao, Xiang
Ying, Tianyi
Wang, Jie
Chen, Huipeng
Wang, Hengliang
author_facet Zhao, Ge
Zhu, Li
Feng, Erling
Cao, Xiaoyu
Shang, Na
Liu, Xiankai
Liao, Xiang
Ying, Tianyi
Wang, Jie
Chen, Huipeng
Wang, Hengliang
author_sort Zhao, Ge
collection PubMed
description BACKGROUND: Shigella flexneri is a gram-negative, facultative pathogen that causes the majority of communicable bacterial dysenteries in developing countries. The virulence factors of S. flexneri have been shown to be produced at 37 degrees C but not at 30 degrees C. To discover potential, novel virulence-related proteins of S. flexneri, we performed differential in-gel electrophoresis (DIGE) analysis to measure changes in the expression profile that are induced by a temperature increase. RESULTS: The ArgT protein was dramatically down-regulated at 37 degrees C. In contrast, the ArgT from the non-pathogenic E. coli did not show this differential expression as in S. flexneri, which suggested that argT might be a potential anti-virulence gene. Competitive invasion assays in HeLa cells and in BALB/c mice with argT mutants were performed, and the results indicated that the over-expression of ArgT(Y225D )would attenuate the virulence of S. flexneri. A comparative proteomic analysis was subsequently performed to investigate the effects of ArgT in S. flexneri at the molecular level. We show that HtrA is differentially expressed among different derivative strains. CONCLUSION: Gene argT is a novel anti-virulence gene that may interfere with the virulence of S. flexneri via the transport of specific amino acids or by affecting the expression of the virulence factor, HtrA.
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spelling pubmed-29047342010-07-16 A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a Zhao, Ge Zhu, Li Feng, Erling Cao, Xiaoyu Shang, Na Liu, Xiankai Liao, Xiang Ying, Tianyi Wang, Jie Chen, Huipeng Wang, Hengliang Proteome Sci Research BACKGROUND: Shigella flexneri is a gram-negative, facultative pathogen that causes the majority of communicable bacterial dysenteries in developing countries. The virulence factors of S. flexneri have been shown to be produced at 37 degrees C but not at 30 degrees C. To discover potential, novel virulence-related proteins of S. flexneri, we performed differential in-gel electrophoresis (DIGE) analysis to measure changes in the expression profile that are induced by a temperature increase. RESULTS: The ArgT protein was dramatically down-regulated at 37 degrees C. In contrast, the ArgT from the non-pathogenic E. coli did not show this differential expression as in S. flexneri, which suggested that argT might be a potential anti-virulence gene. Competitive invasion assays in HeLa cells and in BALB/c mice with argT mutants were performed, and the results indicated that the over-expression of ArgT(Y225D )would attenuate the virulence of S. flexneri. A comparative proteomic analysis was subsequently performed to investigate the effects of ArgT in S. flexneri at the molecular level. We show that HtrA is differentially expressed among different derivative strains. CONCLUSION: Gene argT is a novel anti-virulence gene that may interfere with the virulence of S. flexneri via the transport of specific amino acids or by affecting the expression of the virulence factor, HtrA. BioMed Central 2010-06-12 /pmc/articles/PMC2904734/ /pubmed/20540790 http://dx.doi.org/10.1186/1477-5956-8-30 Text en Copyright ©2010 Zhao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zhao, Ge
Zhu, Li
Feng, Erling
Cao, Xiaoyu
Shang, Na
Liu, Xiankai
Liao, Xiang
Ying, Tianyi
Wang, Jie
Chen, Huipeng
Wang, Hengliang
A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a
title A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a
title_full A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a
title_fullStr A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a
title_full_unstemmed A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a
title_short A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a
title_sort novel anti-virulence gene revealed by proteomic analysis in shigella flexneri 2a
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904734/
https://www.ncbi.nlm.nih.gov/pubmed/20540790
http://dx.doi.org/10.1186/1477-5956-8-30
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