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Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis
PURPOSE: To evaluate the effect of the anti-fibrotic protein serum amyloid P (SAP) on radiation-induced oral mucositis (OM) and fibrosis in a hamster cheek-pouch model. EXPERIMENTAL DESIGN: Hamsters received a single dose of radiation (40 Gy) to the left everted cheek pouch to induce significant OM....
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905325/ https://www.ncbi.nlm.nih.gov/pubmed/20602770 http://dx.doi.org/10.1186/1755-1536-3-11 |
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author | Murray, Lynne A Kramer, Michael S Hesson, David P Watkins, Brynmor A Fey, Edward G Argentieri, Rochelle L Shaheen, Furquan Knight, Darryl A Sonis, Stephen T |
author_facet | Murray, Lynne A Kramer, Michael S Hesson, David P Watkins, Brynmor A Fey, Edward G Argentieri, Rochelle L Shaheen, Furquan Knight, Darryl A Sonis, Stephen T |
author_sort | Murray, Lynne A |
collection | PubMed |
description | PURPOSE: To evaluate the effect of the anti-fibrotic protein serum amyloid P (SAP) on radiation-induced oral mucositis (OM) and fibrosis in a hamster cheek-pouch model. EXPERIMENTAL DESIGN: Hamsters received a single dose of radiation (40 Gy) to the left everted cheek pouch to induce significant OM. The protective therapeutic potential of SAP was evaluated using varying dosing regimens. The extent of OM was measured using a validated six-point scoring scheme ranging from 0 (normal tissue, no mucositis) to 5 (complete ulceration). Fibrotic remodeling was also visualized histologically and quantified at later time points using collagen gene expression. RESULTS: SAP treatment attenuated the profile of radiation-induced oral mucositis by delaying the time of onset, reducing the peak value, and enhancing the resolution of injury. The peak mucositis score was reduced by approximately 0.5 grade in SAP-treated animals. The number of animal days with a score of ≥ 3 was reduced by 48% in the SAP-treated group, compared with the saline control group (P < 0.01). SAP also inhibited the extent of tissue remodeling and decreased radiation-induced increases in myofibroblast number. Attenuated collagen deposition and gene expression was also observed in the cheek pouches of hamsters treated with SAP at both 16 and 28 days post-radiation. CONCLUSIONS: SAP treatment significantly attenuated radiation-induced injury. In particular, SAP attenuated the severity of OM and inhibited pathogenic remodeling. This suggests that SAP may be a useful therapy for the palliation of side effects observed during treatment for head and neck cancer. |
format | Text |
id | pubmed-2905325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29053252010-07-17 Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis Murray, Lynne A Kramer, Michael S Hesson, David P Watkins, Brynmor A Fey, Edward G Argentieri, Rochelle L Shaheen, Furquan Knight, Darryl A Sonis, Stephen T Fibrogenesis Tissue Repair Research PURPOSE: To evaluate the effect of the anti-fibrotic protein serum amyloid P (SAP) on radiation-induced oral mucositis (OM) and fibrosis in a hamster cheek-pouch model. EXPERIMENTAL DESIGN: Hamsters received a single dose of radiation (40 Gy) to the left everted cheek pouch to induce significant OM. The protective therapeutic potential of SAP was evaluated using varying dosing regimens. The extent of OM was measured using a validated six-point scoring scheme ranging from 0 (normal tissue, no mucositis) to 5 (complete ulceration). Fibrotic remodeling was also visualized histologically and quantified at later time points using collagen gene expression. RESULTS: SAP treatment attenuated the profile of radiation-induced oral mucositis by delaying the time of onset, reducing the peak value, and enhancing the resolution of injury. The peak mucositis score was reduced by approximately 0.5 grade in SAP-treated animals. The number of animal days with a score of ≥ 3 was reduced by 48% in the SAP-treated group, compared with the saline control group (P < 0.01). SAP also inhibited the extent of tissue remodeling and decreased radiation-induced increases in myofibroblast number. Attenuated collagen deposition and gene expression was also observed in the cheek pouches of hamsters treated with SAP at both 16 and 28 days post-radiation. CONCLUSIONS: SAP treatment significantly attenuated radiation-induced injury. In particular, SAP attenuated the severity of OM and inhibited pathogenic remodeling. This suggests that SAP may be a useful therapy for the palliation of side effects observed during treatment for head and neck cancer. BioMed Central 2010-07-05 /pmc/articles/PMC2905325/ /pubmed/20602770 http://dx.doi.org/10.1186/1755-1536-3-11 Text en Copyright ©2010 Murray et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Murray, Lynne A Kramer, Michael S Hesson, David P Watkins, Brynmor A Fey, Edward G Argentieri, Rochelle L Shaheen, Furquan Knight, Darryl A Sonis, Stephen T Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis |
title | Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis |
title_full | Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis |
title_fullStr | Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis |
title_full_unstemmed | Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis |
title_short | Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis |
title_sort | serum amyloid p ameliorates radiation-induced oral mucositis and fibrosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905325/ https://www.ncbi.nlm.nih.gov/pubmed/20602770 http://dx.doi.org/10.1186/1755-1536-3-11 |
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