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Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2)
BACKGROUND: The evolutionally conserved MAPK Sty1 and bZIP transcriptional activator Atf1 are known to play a pivotal role in response to the reactive oxygen species in S. pombe. However, it is unclear whether all of the H(2)O(2)-induced genes are directly regulated by the Sty1-Atf1 pathway and invo...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905393/ https://www.ncbi.nlm.nih.gov/pubmed/20661279 http://dx.doi.org/10.1371/journal.pone.0011620 |
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author | Eshaghi, Majid Lee, Jong Hoon Zhu, Lei Poon, Suk Yean Li, Juntao Cho, Kwang-Hyun Chu, Zhaoqing Karuturi, R. Krishna M. Liu, Jianhua |
author_facet | Eshaghi, Majid Lee, Jong Hoon Zhu, Lei Poon, Suk Yean Li, Juntao Cho, Kwang-Hyun Chu, Zhaoqing Karuturi, R. Krishna M. Liu, Jianhua |
author_sort | Eshaghi, Majid |
collection | PubMed |
description | BACKGROUND: The evolutionally conserved MAPK Sty1 and bZIP transcriptional activator Atf1 are known to play a pivotal role in response to the reactive oxygen species in S. pombe. However, it is unclear whether all of the H(2)O(2)-induced genes are directly regulated by the Sty1-Atf1 pathway and involved in growth fitness under H(2)O(2)-induced stress conditions. METHODOLOGY/PRINCIPAL FINDINGS: Here we present the study on ChIP-chip mapping of the genomic binding sites for Sty1, Atf1, and the Atf1's binding partner Pcr1; the genome-wide transcriptional profiling of the atf1 [Image: see text] and pcr1 [Image: see text] strains in response to H(2)O(2); and the phenotypic assessment of ∼90 Atf1/Pcr1-bound or unbound genes for growth fitness under H(2)O(2) conditions. ChIP-chip analysis shows that Atf1 and Pcr1 binding sites are overlapped in the genome and constitutively present before H(2)O(2) stress. On the other hand, Sty1 recruitment primarily occurs at the Atf1/Pcr1 binding sites and is induced by H(2)O(2). We found that Atf1/Pcr1 is clearly responsible for the high-level transcriptional response to H(2)O(2). Furthermore, phenotypic assessment indicates that among the H(2)O(2)-induced genes, Atf1/Pcr1-bound genes exhibit a higher likelihood of functional requirement for growth fitness under the stress condition than the Atf1/Pcr1-unbound genes do. Notably, we found that the Atf1/Pcr1-bound genes regardless of their responsiveness to H(2)O(2) show a high probability of requirement for growth fitness. CONCLUSION/SIGNIFICANCE: Together, our analyses on global mapping of protein binding sites, genome-wide transcriptional profiling, and phenotypic assessment provide insight into mechanisms for global transcriptional regulation by the Sty1-Atf1 pathway in response to H(2)O(2)-induced reactive oxygen species. |
format | Text |
id | pubmed-2905393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29053932010-07-26 Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2) Eshaghi, Majid Lee, Jong Hoon Zhu, Lei Poon, Suk Yean Li, Juntao Cho, Kwang-Hyun Chu, Zhaoqing Karuturi, R. Krishna M. Liu, Jianhua PLoS One Research Article BACKGROUND: The evolutionally conserved MAPK Sty1 and bZIP transcriptional activator Atf1 are known to play a pivotal role in response to the reactive oxygen species in S. pombe. However, it is unclear whether all of the H(2)O(2)-induced genes are directly regulated by the Sty1-Atf1 pathway and involved in growth fitness under H(2)O(2)-induced stress conditions. METHODOLOGY/PRINCIPAL FINDINGS: Here we present the study on ChIP-chip mapping of the genomic binding sites for Sty1, Atf1, and the Atf1's binding partner Pcr1; the genome-wide transcriptional profiling of the atf1 [Image: see text] and pcr1 [Image: see text] strains in response to H(2)O(2); and the phenotypic assessment of ∼90 Atf1/Pcr1-bound or unbound genes for growth fitness under H(2)O(2) conditions. ChIP-chip analysis shows that Atf1 and Pcr1 binding sites are overlapped in the genome and constitutively present before H(2)O(2) stress. On the other hand, Sty1 recruitment primarily occurs at the Atf1/Pcr1 binding sites and is induced by H(2)O(2). We found that Atf1/Pcr1 is clearly responsible for the high-level transcriptional response to H(2)O(2). Furthermore, phenotypic assessment indicates that among the H(2)O(2)-induced genes, Atf1/Pcr1-bound genes exhibit a higher likelihood of functional requirement for growth fitness under the stress condition than the Atf1/Pcr1-unbound genes do. Notably, we found that the Atf1/Pcr1-bound genes regardless of their responsiveness to H(2)O(2) show a high probability of requirement for growth fitness. CONCLUSION/SIGNIFICANCE: Together, our analyses on global mapping of protein binding sites, genome-wide transcriptional profiling, and phenotypic assessment provide insight into mechanisms for global transcriptional regulation by the Sty1-Atf1 pathway in response to H(2)O(2)-induced reactive oxygen species. Public Library of Science 2010-07-16 /pmc/articles/PMC2905393/ /pubmed/20661279 http://dx.doi.org/10.1371/journal.pone.0011620 Text en Eshaghi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Eshaghi, Majid Lee, Jong Hoon Zhu, Lei Poon, Suk Yean Li, Juntao Cho, Kwang-Hyun Chu, Zhaoqing Karuturi, R. Krishna M. Liu, Jianhua Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2) |
title | Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2)
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title_full | Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2)
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title_fullStr | Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2)
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title_full_unstemmed | Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2)
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title_short | Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H(2)O(2)
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title_sort | genomic binding profiling of the fission yeast stress-activated mapk sty1 and the bzip transcriptional activator atf1 in response to h(2)o(2) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905393/ https://www.ncbi.nlm.nih.gov/pubmed/20661279 http://dx.doi.org/10.1371/journal.pone.0011620 |
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