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Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes()
Phosphatidylglycerol and cardiolipin represent the most abundant anionic phospholipid components of cytoplasmic bacterial membranes and thus are used as constituents for membrane mimetic systems. In this study, we have characterized the temperature dependent phase behaviour of the binary system dipa...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Elsevier Science B.V
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905515/ https://www.ncbi.nlm.nih.gov/pubmed/20451316 http://dx.doi.org/10.1016/j.bpc.2010.04.004 |
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author | Prossnigg, Florian Hickel, Andrea Pabst, Georg Lohner, Karl |
author_facet | Prossnigg, Florian Hickel, Andrea Pabst, Georg Lohner, Karl |
author_sort | Prossnigg, Florian |
collection | PubMed |
description | Phosphatidylglycerol and cardiolipin represent the most abundant anionic phospholipid components of cytoplasmic bacterial membranes and thus are used as constituents for membrane mimetic systems. In this study, we have characterized the temperature dependent phase behaviour of the binary system dipalmitoyl-phosphatidylglycerol (DPPG) and tetramyristoyl-cardiolipin (TMCL) using microcalorimetry and X-ray scattering techniques. Both lipids exhibited a very similar main transition temperature (∼ 41 °C), showing a minimum (39.4 °C) for the binary mixtures at X(DPPG) = 0.8, and exhibited low-temperature phase transitions, which were abolished by incorporation of small amounts (≤ 10 mol%) of the other lipid component. Therefore, over a wide temperature and composition range a lamellar L(β) gel phase is the predominant structure below the chain melting transition, characterized by a relatively broad wide-angle peak for X(DPPG) ≤ 0.8. This observation suggests the existence of packing inconsistencies of the TMCL/DPPG hydrocarbon lattices in the gel phase, supported by the small average size of lipid clusters (∼ 50 lipids) within this composition range. The bilayer thickness for the lamellar-gel phase showed a monotonic increase (56 Å for TMCL to about 58 Å for X(DPPG) = 0.8 at 30 °C), which may be explained by different degrees of partial interdigitation of the acyl chains to compensate for the differences in the hydrocarbon lengths of DPPG and TMCL in the L(β) phase. |
format | Text |
id | pubmed-2905515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Elsevier Science B.V |
record_format | MEDLINE/PubMed |
spelling | pubmed-29055152010-08-04 Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() Prossnigg, Florian Hickel, Andrea Pabst, Georg Lohner, Karl Biophys Chem Article Phosphatidylglycerol and cardiolipin represent the most abundant anionic phospholipid components of cytoplasmic bacterial membranes and thus are used as constituents for membrane mimetic systems. In this study, we have characterized the temperature dependent phase behaviour of the binary system dipalmitoyl-phosphatidylglycerol (DPPG) and tetramyristoyl-cardiolipin (TMCL) using microcalorimetry and X-ray scattering techniques. Both lipids exhibited a very similar main transition temperature (∼ 41 °C), showing a minimum (39.4 °C) for the binary mixtures at X(DPPG) = 0.8, and exhibited low-temperature phase transitions, which were abolished by incorporation of small amounts (≤ 10 mol%) of the other lipid component. Therefore, over a wide temperature and composition range a lamellar L(β) gel phase is the predominant structure below the chain melting transition, characterized by a relatively broad wide-angle peak for X(DPPG) ≤ 0.8. This observation suggests the existence of packing inconsistencies of the TMCL/DPPG hydrocarbon lattices in the gel phase, supported by the small average size of lipid clusters (∼ 50 lipids) within this composition range. The bilayer thickness for the lamellar-gel phase showed a monotonic increase (56 Å for TMCL to about 58 Å for X(DPPG) = 0.8 at 30 °C), which may be explained by different degrees of partial interdigitation of the acyl chains to compensate for the differences in the hydrocarbon lengths of DPPG and TMCL in the L(β) phase. Elsevier Science B.V 2010-08 /pmc/articles/PMC2905515/ /pubmed/20451316 http://dx.doi.org/10.1016/j.bpc.2010.04.004 Text en © 2010 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license |
spellingShingle | Article Prossnigg, Florian Hickel, Andrea Pabst, Georg Lohner, Karl Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
title | Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
title_full | Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
title_fullStr | Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
title_full_unstemmed | Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
title_short | Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
title_sort | packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905515/ https://www.ncbi.nlm.nih.gov/pubmed/20451316 http://dx.doi.org/10.1016/j.bpc.2010.04.004 |
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