Accurate non-invasive image-based cytotoxicity assays for cultured cells

BACKGROUND: The CloneSelect™ Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay. RESULTS: Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC(50 )values w...

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Autores principales: Marqués-Gallego, Patricia, den Dulk, Hans, Backendorf, Claude, Brouwer, Jaap, Reedijk, Jan, Burke, Julian F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2906423/
https://www.ncbi.nlm.nih.gov/pubmed/20565747
http://dx.doi.org/10.1186/1472-6750-10-43
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author Marqués-Gallego, Patricia
den Dulk, Hans
Backendorf, Claude
Brouwer, Jaap
Reedijk, Jan
Burke, Julian F
author_facet Marqués-Gallego, Patricia
den Dulk, Hans
Backendorf, Claude
Brouwer, Jaap
Reedijk, Jan
Burke, Julian F
author_sort Marqués-Gallego, Patricia
collection PubMed
description BACKGROUND: The CloneSelect™ Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay. RESULTS: Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC(50 )values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay. CONCLUSIONS: This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.
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spelling pubmed-29064232010-07-20 Accurate non-invasive image-based cytotoxicity assays for cultured cells Marqués-Gallego, Patricia den Dulk, Hans Backendorf, Claude Brouwer, Jaap Reedijk, Jan Burke, Julian F BMC Biotechnol Methodology Article BACKGROUND: The CloneSelect™ Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay. RESULTS: Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC(50 )values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay. CONCLUSIONS: This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested. BioMed Central 2010-06-17 /pmc/articles/PMC2906423/ /pubmed/20565747 http://dx.doi.org/10.1186/1472-6750-10-43 Text en Copyright ©2010 Marqués-Gallego et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Marqués-Gallego, Patricia
den Dulk, Hans
Backendorf, Claude
Brouwer, Jaap
Reedijk, Jan
Burke, Julian F
Accurate non-invasive image-based cytotoxicity assays for cultured cells
title Accurate non-invasive image-based cytotoxicity assays for cultured cells
title_full Accurate non-invasive image-based cytotoxicity assays for cultured cells
title_fullStr Accurate non-invasive image-based cytotoxicity assays for cultured cells
title_full_unstemmed Accurate non-invasive image-based cytotoxicity assays for cultured cells
title_short Accurate non-invasive image-based cytotoxicity assays for cultured cells
title_sort accurate non-invasive image-based cytotoxicity assays for cultured cells
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2906423/
https://www.ncbi.nlm.nih.gov/pubmed/20565747
http://dx.doi.org/10.1186/1472-6750-10-43
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AT backendorfclaude accuratenoninvasiveimagebasedcytotoxicityassaysforculturedcells
AT brouwerjaap accuratenoninvasiveimagebasedcytotoxicityassaysforculturedcells
AT reedijkjan accuratenoninvasiveimagebasedcytotoxicityassaysforculturedcells
AT burkejulianf accuratenoninvasiveimagebasedcytotoxicityassaysforculturedcells

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