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The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei

BACKGROUND: Knowledge of the virus-host cell interaction could inform us of the molecular pathways exploited by the virus. Studies on viral attachment proteins (VAPs) and candidate receptor proteins involved in WSSV infection, allow a better understanding of how these proteins interact in the viral...

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Autores principales: Liang, Yan, Cheng, Jun-Jun, Yang, Bing, Huang, Jie
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2906456/
https://www.ncbi.nlm.nih.gov/pubmed/20591132
http://dx.doi.org/10.1186/1743-422X-7-144
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author Liang, Yan
Cheng, Jun-Jun
Yang, Bing
Huang, Jie
author_facet Liang, Yan
Cheng, Jun-Jun
Yang, Bing
Huang, Jie
author_sort Liang, Yan
collection PubMed
description BACKGROUND: Knowledge of the virus-host cell interaction could inform us of the molecular pathways exploited by the virus. Studies on viral attachment proteins (VAPs) and candidate receptor proteins involved in WSSV infection, allow a better understanding of how these proteins interact in the viral life cycle. In this study, our aim was to find some host cellular membrane proteins that could bind with white spot syndrome virus (WSSV). RESULTS: Two proteins were evident by using a virus overlay protein binding assay (VOPBA) with WSSV. A protein with molecular weight 53 kDa, named BP53, was analyzed in this study, which was homologous with the F(1)-ATP synthase beta subunit by mass spectrometry analysis. Rapid amplification of cDNA ends (RACE) PCR was performed to identify the full-length cDNA of the bp53 gene. The resulting full-length gene consisted of 1836 bp, encoding 525 amino acids with a calculated molecular mass of 55.98 kDa. The deduced amino acid sequence contained three conserved domains of the F(1)-ATP synthase beta subunit. BP53 was therefore designated the F(1)-ATP synthase beta subunit of L. vannamei. The binding of WSSV to BP53 were also confirmed by competitive ELISA binding assay and co-immunoprecipitation on magnetic beads. To investigate the function of BP53 in WSSV infection, it was mixed with WSSV before the mixture was injected intramuscularly into shrimp. The resulting mortality curves showed that recombinant (r) BP53 could attenuate WSSV infection. CONCLUSIONS: The results revealed that BP53 is involved in WSSV infection. Here is the first time showed the role of shrimp F(1)-ATP synthase beta subunit in WSSV infection.
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spelling pubmed-29064562010-07-20 The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei Liang, Yan Cheng, Jun-Jun Yang, Bing Huang, Jie Virol J Research BACKGROUND: Knowledge of the virus-host cell interaction could inform us of the molecular pathways exploited by the virus. Studies on viral attachment proteins (VAPs) and candidate receptor proteins involved in WSSV infection, allow a better understanding of how these proteins interact in the viral life cycle. In this study, our aim was to find some host cellular membrane proteins that could bind with white spot syndrome virus (WSSV). RESULTS: Two proteins were evident by using a virus overlay protein binding assay (VOPBA) with WSSV. A protein with molecular weight 53 kDa, named BP53, was analyzed in this study, which was homologous with the F(1)-ATP synthase beta subunit by mass spectrometry analysis. Rapid amplification of cDNA ends (RACE) PCR was performed to identify the full-length cDNA of the bp53 gene. The resulting full-length gene consisted of 1836 bp, encoding 525 amino acids with a calculated molecular mass of 55.98 kDa. The deduced amino acid sequence contained three conserved domains of the F(1)-ATP synthase beta subunit. BP53 was therefore designated the F(1)-ATP synthase beta subunit of L. vannamei. The binding of WSSV to BP53 were also confirmed by competitive ELISA binding assay and co-immunoprecipitation on magnetic beads. To investigate the function of BP53 in WSSV infection, it was mixed with WSSV before the mixture was injected intramuscularly into shrimp. The resulting mortality curves showed that recombinant (r) BP53 could attenuate WSSV infection. CONCLUSIONS: The results revealed that BP53 is involved in WSSV infection. Here is the first time showed the role of shrimp F(1)-ATP synthase beta subunit in WSSV infection. BioMed Central 2010-06-30 /pmc/articles/PMC2906456/ /pubmed/20591132 http://dx.doi.org/10.1186/1743-422X-7-144 Text en Copyright ©2010 Liang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Liang, Yan
Cheng, Jun-Jun
Yang, Bing
Huang, Jie
The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
title The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
title_full The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
title_fullStr The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
title_full_unstemmed The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
title_short The role of F(1 )ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
title_sort role of f(1 )atp synthase beta subunit in wssv infection in the shrimp, litopenaeus vannamei
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2906456/
https://www.ncbi.nlm.nih.gov/pubmed/20591132
http://dx.doi.org/10.1186/1743-422X-7-144
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