Inositol 1,4,5-trisphosphate-induced Ca(2+ )signalling is involved in estradiol-induced breast cancer epithelial cell growth

BACKGROUND: Ca(2+ )is a ubiquitous messenger that has been shown to be responsible for controlling numerous cellular processes including cell growth and cell death. Whereas the involvement of IP(3)-induced Ca(2+ )signalling (IICS) in the physiological activity of numerous cell types is well documented, the role of IICS in cancer cells is still largely unknown. Our purpose was to characterize the role of IICS in the control of growth of the estrogen-dependent human breast cancer epithelial cell line MCF-7 and its potential regulation by 17β-estradiol (E(2)). RESULTS: Our results show that the IP(3 )receptor (IP(3)R) inhibitors caffeine, 2-APB and xestospongin C (XeC) inhibited the growth of MCF-7 stimulated by 5% foetal calf serum or 10 nM E(2). Furthermore, Ca(2+ )imaging experiments showed that serum and E(2 )were able to trigger, in a Ca(2+)-free medium, an elevation of internal Ca(2+ )in a 2-APB and XeC-sensitive manner. Moreover, the phospholipase C (PLC) inhibitor U-73122 was able to prevent intracellular Ca(2+ )elevation in response to serum, whereas the inactive analogue U-73343 was ineffective. Western-blotting experiments revealed that the 3 types of IP(3)Rs are expressed in MCF-7 cells and that a 48 hours treatment with 10 nM E(2 )elevated IP(3)R3 protein expression level in an ICI-182,780 (a specific estrogen receptor antagonist)-dependent manner. Furthermore, IP(3)R3 silencing by the use of specific small interfering RNA was responsible for a drastic modification of the temporal feature of IICS, independently of a modification of the sensitivity of the Ca(2+ )release process and acted to counteract the proliferative effect of 10 nM E(2). CONCLUSIONS: Altogether, our results are in favour of a role of IICS in MCF-7 cell growth, and we hypothesize that the regulation of IP(3)R3 expression by E(2 )is involved in this effect.