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An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer

BACKGROUND: Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse. METHODS: We included 153 patients with stage I-II hormonal receptor-pos...

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Autores principales: Sánchez-Navarro, Iker, Gámez-Pozo, Angelo, Pinto, Álvaro, Hardisson, David, Madero, Rosario, López, Rocío, San José, Belén, Zamora, Pilar, Redondo, Andrés, Feliu, Jaime, Cejas, Paloma, González Barón, Manuel, Ángel Fresno Vara, Juan, Espinosa, Enrique
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2906483/
https://www.ncbi.nlm.nih.gov/pubmed/20584321
http://dx.doi.org/10.1186/1471-2407-10-336
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author Sánchez-Navarro, Iker
Gámez-Pozo, Angelo
Pinto, Álvaro
Hardisson, David
Madero, Rosario
López, Rocío
San José, Belén
Zamora, Pilar
Redondo, Andrés
Feliu, Jaime
Cejas, Paloma
González Barón, Manuel
Ángel Fresno Vara, Juan
Espinosa, Enrique
author_facet Sánchez-Navarro, Iker
Gámez-Pozo, Angelo
Pinto, Álvaro
Hardisson, David
Madero, Rosario
López, Rocío
San José, Belén
Zamora, Pilar
Redondo, Andrés
Feliu, Jaime
Cejas, Paloma
González Barón, Manuel
Ángel Fresno Vara, Juan
Espinosa, Enrique
author_sort Sánchez-Navarro, Iker
collection PubMed
description BACKGROUND: Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse. METHODS: We included 153 patients with stage I-II hormonal receptor-positive breast cancer. RNA was isolated from formalin-fixed paraffin-embedded samples and qRT-PCR amplification of 83 genes was performed with gene expression assays. The genes we analyzed were those included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Index. The association among gene expression, clinical variables and distant metastasis-free survival was analyzed using Cox regression models. RESULTS: An 8-gene prognostic score was defined. Distant metastasis-free survival at 5 years was 97% for patients defined as low-risk by the prognostic score versus 60% for patients defined as high-risk. The 8-gene score remained a significant factor in multivariate analysis and its performance was similar to that of two validated gene profiles: the 70-Gene Signature and the Recurrence Score. The validity of the signature was verified in independent cohorts obtained from the GEO database. CONCLUSIONS: This study identifies a simple gene expression score that complements histopathological prognostic factors in breast cancer, and can be determined in paraffin-embedded samples.
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spelling pubmed-29064832010-07-20 An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer Sánchez-Navarro, Iker Gámez-Pozo, Angelo Pinto, Álvaro Hardisson, David Madero, Rosario López, Rocío San José, Belén Zamora, Pilar Redondo, Andrés Feliu, Jaime Cejas, Paloma González Barón, Manuel Ángel Fresno Vara, Juan Espinosa, Enrique BMC Cancer Research Article BACKGROUND: Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse. METHODS: We included 153 patients with stage I-II hormonal receptor-positive breast cancer. RNA was isolated from formalin-fixed paraffin-embedded samples and qRT-PCR amplification of 83 genes was performed with gene expression assays. The genes we analyzed were those included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Index. The association among gene expression, clinical variables and distant metastasis-free survival was analyzed using Cox regression models. RESULTS: An 8-gene prognostic score was defined. Distant metastasis-free survival at 5 years was 97% for patients defined as low-risk by the prognostic score versus 60% for patients defined as high-risk. The 8-gene score remained a significant factor in multivariate analysis and its performance was similar to that of two validated gene profiles: the 70-Gene Signature and the Recurrence Score. The validity of the signature was verified in independent cohorts obtained from the GEO database. CONCLUSIONS: This study identifies a simple gene expression score that complements histopathological prognostic factors in breast cancer, and can be determined in paraffin-embedded samples. BioMed Central 2010-06-28 /pmc/articles/PMC2906483/ /pubmed/20584321 http://dx.doi.org/10.1186/1471-2407-10-336 Text en Copyright ©2010 Sánchez-Navarro et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sánchez-Navarro, Iker
Gámez-Pozo, Angelo
Pinto, Álvaro
Hardisson, David
Madero, Rosario
López, Rocío
San José, Belén
Zamora, Pilar
Redondo, Andrés
Feliu, Jaime
Cejas, Paloma
González Barón, Manuel
Ángel Fresno Vara, Juan
Espinosa, Enrique
An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
title An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
title_full An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
title_fullStr An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
title_full_unstemmed An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
title_short An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
title_sort 8-gene qrt-pcr-based gene expression score that has prognostic value in early breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2906483/
https://www.ncbi.nlm.nih.gov/pubmed/20584321
http://dx.doi.org/10.1186/1471-2407-10-336
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