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Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?

PURPOSE: To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. MATERIALS AND METHODS: Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient a...

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Autores principales: Das, Sujata, Sharma, Savitri, Kar, Sarita, K Sahu, Srikant, Samal, Bikash, Mallick, Aparajita
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907027/
https://www.ncbi.nlm.nih.gov/pubmed/20534916
http://dx.doi.org/10.4103/0301-4738.64122
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author Das, Sujata
Sharma, Savitri
Kar, Sarita
K Sahu, Srikant
Samal, Bikash
Mallick, Aparajita
author_facet Das, Sujata
Sharma, Savitri
Kar, Sarita
K Sahu, Srikant
Samal, Bikash
Mallick, Aparajita
author_sort Das, Sujata
collection PubMed
description PURPOSE: To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. MATERIALS AND METHODS: Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with Escherichia coli overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. RESULTS: Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, P = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in in vitro experiment although the characteristic spores and color were seen earlier on SDA. CONCLUSION: Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.
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spelling pubmed-29070272010-08-02 Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? Das, Sujata Sharma, Savitri Kar, Sarita K Sahu, Srikant Samal, Bikash Mallick, Aparajita Indian J Ophthalmol Original Article PURPOSE: To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. MATERIALS AND METHODS: Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with Escherichia coli overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. RESULTS: Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, P = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in in vitro experiment although the characteristic spores and color were seen earlier on SDA. CONCLUSION: Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. Medknow Publications 2010 /pmc/articles/PMC2907027/ /pubmed/20534916 http://dx.doi.org/10.4103/0301-4738.64122 Text en © Indian Journal of Ophthalmology http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Das, Sujata
Sharma, Savitri
Kar, Sarita
K Sahu, Srikant
Samal, Bikash
Mallick, Aparajita
Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
title Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
title_full Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
title_fullStr Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
title_full_unstemmed Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
title_short Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
title_sort is inclusion of sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907027/
https://www.ncbi.nlm.nih.gov/pubmed/20534916
http://dx.doi.org/10.4103/0301-4738.64122
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